Reagent for RNA/DNA/protein stabilization from PBMC?

More Sylvain Monnier-Benoit's questions See All

Could anyone know any application which allows to be able to determine the modal composition of a rock from its whole rock chemical composition?

I have to classify some rocks using their modal composition, but no point counter in my disposal, that's why I would like to know if some one may help me with any Excel sheet or reliable...

31 October 2023 1,280 3 View

Guidance needed : 3D ceramic printing what do you think?

We have a project in the field of ceramic 3D printing for which we are currently analyzing the technical interest and potential markets. Below is the link to the feedback platform, where you...

24 August 2023 7,142 0 View

In Construction Grammar, how frequent is frequent enough?

It's all in the question, really. This topic must have been raised and discussed before, but I can't seem to find a good reference just yet. I'm writing this paper where I make the case for a...

20 February 2023 2,552 6 View

Apotracker Green with other surface antibodies for flow cytometry ?

Hi, I would like to use Apotracker green (Biolegend) with other surface antibodies. Does someone have experience with it ? Would you add the Apotracker Green together with the other antibody or...

13 January 2023 9,084 3 View

Which French diagnostic questionnaire can i use to attest bipolar trouble diagnosis for a study ?

Hello, Most research on bipolar disorder are in English and use The SCID-IV questionnaire to attest the diagnosis. Can anyone refer me a questionnaire in French with a good validity for a...

07 September 2022 9,946 0 View

Does anyone know a repository of attack patterns on network packet captures?

I am testing a new intrusion detection system and I need samples of traffic (network packet captures) along with signatures (i.e. patterns) of attacks that can be detected on this traffic. Does...

24 August 2022 7,681 0 View

Is there an efficient way to degrade biotin ?

I want to degrade biotin after proximity labelling with turboID but found nothing about a commercial enzyme or chemical dedicated to this task. Does anyone know if there is a simple mean to do...

29 March 2022 1,019 3 View

How to import netCDF files without coordinates in GIS?

Hey, I want to imported photosynthetically available radiation (PAR) data from Gentili and Gattuso (2020) available here as netCDF format (https://doi.pangaea.de/10.1594/PANGAEA.910898). The...

15 March 2022 2,461 0 View

Compute number of unique choice tasks and second question how to code Alternative specific constant?

Hello, I have two small questions for people experienced in discrete choice experiments and use of mixed logit models. I am currently conducting a DCE with forced choices with 3 alternatives...

04 January 2022 6,352 6 View

How to get wind data for multiple altitudes?

I need wind speed data for a various altitudes (at least till to 1000m) in brazilian south region.

08 April 2021 9,262 3 View

Is there a problem with my RNA pellet?

Hello, I am currently having problems with RNA extraction. I am using mouse liver (C57BL6J), and I have extracted RNA from mouse liver before. Before this experiment, my final RNA pellets were...

11 August 2024 7,082 3 View

Strugglling with m6A dot blot any suugesstion ?

I have been doing the m6A dot blot for a while with no improvement, I am extracting the RNA, and I can see the dots although the three biological replicas give a different reading on the memberan...

10 August 2024 8,539 5 View

Why Do TDS and EC Increase with Larger Wastewater Volumes, While BOD and COD Decrease?

I have carried out MFC experiments on three different volumes, 50, 500 and 1000 mL of wastewater. Results after MFC treatment shows that TDS and EC are more in larger volumes of water i.e. TDS and...

09 August 2024 9,621 0 View

Could it be a cell culture contamination?

Hello everyone! I observed in my culture (htert-RPE1 cells) an orange- red particle at the bottom of the dish. It is visible to the naked eye as a very very small red dot. Could it be a...

09 August 2024 2,824 3 View

RNA Extraction Using Hot Borate Method No Longer Working?

I've been performing RNA extraction on cotton petiole tissue for a few months now using the method described in the following paper, a derivative of the typical hot borate method...

08 August 2024 9,882 2 View

Does Anyone have expertise in in vitro transcription and RNA pull down assay?

I am currently working on LncRNA; to know the lncRNA-protein interactions I want to do RNA pull down assay, so I need to design primers with T7 promoter. I need assistance in this regard.

07 August 2024 6,622 1 View

Is there any way to quantify bacterial and fungal cells in their mixed culture?

I am working in fungal fermentation of soybean meal and there is bacterial growth in them at times. I am trying to quantify fungal cell counts and bacterial cells; but I haven't been able to do at...

07 August 2024 7,535 4 View

How to normalize and take the significance of the MTT OD values with 3 replicates for the same cell-line?

Hi, I have a question about normalizing the MTT OD values for doing the statistical analysis. So, if we have 3 different plates and we call them 3 different replicates, so, first we would...

07 August 2024 8,106 4 View

Why activated CAR-Jurkat cell could not kill targets?

Previously when I co-coluture anti-CD19(FMC63) CAR-Jurkat with Raji with E:T=5:1, Jurkat can eliminate Raji in 24h. However, when I test another CAR construct, although I can dectect totally CD69...

06 August 2024 641 2 View

Why does my protein refolded to beta sheet during thermal denaturation analysis?

Hi! So i attempted to understand a novel protein behavior towards heat application by analyzing its secondary structure change. I subjected the protein to a thermal denaturation analysis using...

06 August 2024 1,989 3 View

Shamsher S. Kanwar

You may consider using a proteases inhibitor cocktail as well as DEPC [Diethyl pyrocarbonate] that is RNase inhibitor.

Sylvain Monnier-Benoit

Thank you for the answer. I'm searching a commercial solution, like RNAlater or others. I have mitigated experience with RNAlater on PBMC, due to the difficulty to pellet the cells before extraction, and adding RLT buffer (lysis).

Jack Gan

Hi Sylvain,

Have you considered DNA/RNA Shield? You can find information about it here: https://www.zymoresearch.com/pages/dna-rna-shield

If you have any further questions about it, let me know and perhaps we can connect.

Best,

Jack