rDNA Technology in current scenarios requires a new revolution. Because of tumor cell heterogeneity with in a tumor it very difficult to detect cancer cell.Concept of cancer stem cell added another dilemma in cancer biology,but as a natural rule of the cell most of fuctions are govern by change in gene expression and mutation .Overall it now requires Next -Generation Technology,which have immense capacity to sequence genome in less time and cost effective manner .
But again comes the question of accuracy and data analysis to translate this info for the patients.
solution are there and solved by bioinformatics software's.
Hopefully ,the development of RNAi technology and miRNA approach will help to decipher the mystery of cancer by the use of rDNA technology.