I would like to know if anyone has issue with the Corning osteo assay surface.
The RAW264.7 cannot form muti-nucleated osteoclast using the osteo assay, but it can differentiate on a regular plate. Same medium (50 & 100ng/ml RANKL) was used and changed every 3 days
On the osteo assay surface, the cells do proliferate but they do not seems to be differentiating. No resorption pit was found after removing the cells by bleaching. I have also tried different seeding density from 1000 to 30 cell/well (96 well plate), the result remains the same.
I seen other papers successfully differentiate RAW264.7 on osteo assay. May I ask am I dong something wrong here?