We have now tried a number of commercially available antibodies to detect the receptor of AGEs (RAGE, sometimes called AGER) in parrafin embedded mouse tissue with DAB staining. As a control we have used corresponding tissues of a RAGE-KO mouse. Whereas most of these antibodies worked well in lung tissue that accumulates RAGE to a high amount, we have faced significant problems when using tissues that express only minor amounts of RAGE. In particular we observed cross reactivity with muscle cells. If anybody has had good results using an anti RAGE antibody for IHC in mouse tissue please let us know!
this one gives good results on mouse lung (IHC and WB), but I never used it with RAGE ko tissues.
http://www.antibodies-biologo.com/Antikoerper/AGE/RAGE001/976.html
Did you finally found one that worked well in other tissues? I would like to check RAGE in pfa-fixed mouse brain slices.
Thanks!
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