I am looking for cell lines sensitive to irradiation (X-rays) to use as a control. Does anyone have any experience in working with cells that 'die readily' and show signs of apoptosis upon irradiation? Thanks.
Having worked only with primary cells, I wouldn't really be capable of answering your question. I could only suggest to use cells of lymphoid lineage, since apoptosis is their "preferred" way of dying … Primary human lymphocytes, dependent on their actual phenotype, readily declare apoptosis, as of 12 hours after 0.5 Gy irradiation.
HTH
Human cell lines HL-60 (p53 null myeloid leukemia), Molt-4 (p53 wild-type T-lymphoblastoid) and U937 (p53 null myelomonocytic), and Jurkat (p53 null acute T lymphoblastic leukemia) are highly sensitive to ionizing radiation-induced apoptosis. Broadly, typical radioresponsive tumors include seminoma, germinoma, leukemia, lymphoma, Wilms tumor, and non-melanoma skin cancer, and typical normal tissues sensitive to ionizing radiation induced apoptosis include lymphocytes, thymus, and spleen. However, apoptosis is not a major mode of cell death in various cell types, and therefore does not necessarily account for radiosensitivity.
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