What happens with the fluorescence lifetime if the nanoparticles' surface has been modified or exchanged with a new ligand?

Oleic acid ligand on as prepared nanoparticles (dispersed in cyclohexane) has been exchanged by the citric acid ligand with -COOH terminated at its end and could be dispersed in water. I am just...

09 October 2017 9,611 0 View

Prepertion protocol for citrate bufer (pH 3-6), and phosphate buffer (7-8).

07 August 2017 1,229 3 View

Key differences between excitation and absorption.

I am very curious to know what is or are the key difference(s) between absorption and excitation of a molecule (fluorescence). I would like to have more like a general statement about the...

05 June 2017 6,935 3 View

Which experiment would be suitable to find if nanoparticles' surface has been exchanged with new ligands ?

I am attempting to exchange nanoparticle's surface ligand (oleic acid) with another ligand (i.e., citric acid). However, the product yield is too small to get it in powder form and not enough to...

04 May 2017 5,335 13 View

How can I purify mOrange protein after it expressed from E.Coli?

08 September 2016 7,286 3 View

Definition of Positive and Negative Photo-response

We fabricated two Photoconductor type MoS2 based Photodetector device. In one we noticed Positive photoresponse and Negative photoresponse on the other. How to define and explain these two...

01 January 1970 1,586 8 View

What happen to the doubling time of old generation (54th) HeLa cells?

I intend to start working on HeLa cells and I read online that, the doubling time of HeLa cells is ~20.67 hrs. But, meanwhile, I was told by a colleague of mine that when HeLa cells grows old...

01 January 1970 5,642 6 View

I need to image mesoporous silica nanoparticles that are in ~100 nm size and do high resolution TEM to look at mesopores. what grids should I use?

Need to image mesoporous silica nanoparticles using the TEM. Also, need high resolution TEM images to see the mesoporous structure. Kindly suggest what kind of grids to use. Thanks, Shatadru

02 March 2021 1,787 2 View

Can chitosan alone can act as a reducing agent for Cu(0) nanoparticle sythesis?

[Synthesizing Cu nanoparticles without using chemical reductants]

01 March 2021 8,359 1 View

How to simultaneously perform multiple biochemical assays?

I am a research scholar working on heavy metal stress on plants. I will be doing biochemical characterisation( protein, carbohydrate, proline, antioxidant enzymes and many more assays) at interval...

01 March 2021 6,999 3 View

What are the new 2D nanomaterials were used for Silver nanoparticles stabilization?

In the stabilization of AgNPs are vital in current research interest. However, some of the 2D nano-materials like graphene oxide, MoS2, etc... previously used to stabilize it. But still they shown...

01 March 2021 2,207 3 View

What is the state of the drug inside solid lipid nanoparticles (SLNs)?

I am a little confuse with the mechanism of solid lipid nanoparticles (SLNs). The main point of encapsulating drugs into SLNs is because some drugs have low solubility in water. Therefore, SLNs...

01 March 2021 3,703 3 View

How to check the uv-vis absorption of nanoparticle by surface plasmon resonance?

I try to check the uv-vis absorption of Indium nanoparticle. Now I am measuring in solution, can I measure it in the form of a film?

01 March 2021 930 3 View

Distribution of particles in metal matrix?

How would define the "good" and "poor" distribution of particles within the matrix. Is there a quantitative solution to assess the distribution of the particles, which then having denotation of...

28 February 2021 7,397 8 View

How can I calculate the rdf of water molecules around 'Na' ions that are closest(adsorbed) to the nanoparticle(solute)?

Hi, I want to calculate the radial distribution function for water molecules around the Na+ ions adsorbed on the surface of a spherical nanoparticle using the gromacs trajectory dynamically? For...

28 February 2021 5,650 1 View

How to calculate Ligand-active site distance in a ligand-protein complex from MD simulation?

I have run a MD simulation of a ligand-protein complex in GROMACS using CHARMM forcefield. Reviewer has asked me to calculate Ligand-activesite distance and binding free energy of ligand with...

27 February 2021 4,703 1 View

LEDs on Vis Light photo degradation of MB water sol via N doped TiO2 in a mini flume?

Good day to all, I have no experience with light emitting diodes. I am building a glass mini flume to demonstrate the photo degradation of Methylene Blue in water running over self cleaning...

26 February 2021 4,026 5 View