Sandip Ghosh

8 Questions 10 Answers 0 Followers

Questions related from Sandip Ghosh

What happens with the fluorescence lifetime if the nanoparticles' surface has been modified or exchanged with a new ligand?

Oleic acid ligand on as prepared nanoparticles (dispersed in cyclohexane) has been exchanged by the citric acid ligand with -COOH terminated at its end and could be dispersed in water. I am just...

10 October 2017 9,683 0 View

Prepertion protocol for citrate bufer (pH 3-6), and phosphate buffer (7-8).

Hi everyone, I want to prepare following two different buffer with different pH stock (20 ml each). 1. Citrate buffer (pH: 3, 4, 5, and 6) 2. Phosphate buffer (pH: 7, 8) I would be grateful if...

08 August 2017 1,300 3 View

Key differences between excitation and absorption.

I am very curious to know what is or are the key difference(s) between absorption and excitation of a molecule (fluorescence). I would like to have more like a general statement about the...

06 June 2017 7,048 3 View

Which experiment would be suitable to find if nanoparticles' surface has been exchanged with new ligands ?

I am attempting to exchange nanoparticle's surface ligand (oleic acid) with another ligand (i.e., citric acid). However, the product yield is too small to get it in powder form and not enough to...

05 May 2017 5,404 13 View

How can I purify mOrange protein after it expressed from E.Coli?

I have transformed and expressing his tagged-mOrange protein in BL21 strains, later I am expecting the high purity(99-100%) mOrange protein without having changed in protein configuration. So,...

09 September 2016 7,355 3 View

"searching for a ligand from metal to upconversion nanoparticles", could anybody guide me to the right way to find these kind of ligand?

keywords: lanthanidesligands metals

08 August 2016 2,114 0 View

Definition of Positive and Negative Photo-response

We fabricated two Photoconductor type MoS2 based Photodetector device. In one we noticed Positive photoresponse and Negative photoresponse on the other. How to define and explain these two...

01 January 1970 1,658 8 View

What happen to the doubling time of old generation (54th) HeLa cells?

I intend to start working on HeLa cells and I read online that, the doubling time of HeLa cells is ~20.67 hrs. But, meanwhile, I was told by a colleague of mine that when HeLa cells grows old...

01 January 1970 5,713 6 View