Defining mixed model with repeated measures data?

HI all, I have been working with daily diaries data ( 6 emotions of which 3 positive and 3 negative) were assessed for 30 days. These observations (1level data) are then nested within subjects...

13 February 2021 1,810 3 View

High percentage agarose gel for better resolution?

Hi all, it might be a very basic question, but I need some opinions: I need to separate a 294 bp wild type PCR product from a 232 bp knock-out PCR product. I want to do DNA extraction out of the...

08 June 2020 9,557 9 View

What do you think of the impact of Covid-19 on faster acceptance of innovative healthcare technologies?

After this pandemic outbreak, nothing will be the same. Previously suspicious attitude toward accepting innovative solutions in clinical practice and long times for regulatory procedures and...

09 April 2020 7,691 10 View

Any experience with Health Informatics Journal?

We would like to publish something which is in the domain of Health Informatics, but several Journals have almost identical names. any suggestions, experience with publishing with those Journals...

22 February 2020 7,635 9 View

Do we need a standardization prior to publication of any machine learning application?

What do you think about so high accuracies reported in so many publications especially in medicine and psychiatry? Talking about overfitting and so frequent lack of internal and external...

07 October 2019 2,558 4 View

Is it possible to stain cells with Acridine orange/ethidium bromide in hydrogel 3D invasion assay?

I am planning to perform 3D invasion assay with spheroids in hydrogel and to visualise live/dead cells. Is it possible to visualise cells with AO/EB (besides CAM/EtHD) dyes? Thanks

18 October 2018 5,722 3 View

Can LPS be captivated by the tube if you freeze and subsequently thaw it?

15 January 2017 7,051 3 View

Does someone can recommend a good Elisa kit for mouse NTx (N-terminal telopeptide ) ?

Hello everyone, I need to measure NTx (N-terminal telopeptide) marker in a mouse serum. I would really appreciate if someone could share experience with this kind of ELISA kit, most importantly...

10 April 2016 5,458 1 View

What is wrong with my intestinal organoids?

I am trying to grow swine intestinal organoids from crypt preps. I have been doing it with mouse tissue for at least a year now. For the pigs, a few days after getting the crypts, there are...

05 April 2016 3,294 7 View

Does anyone know how to create nucleotide and amino acid deduced sequence visualization in an automated way?

I would like to generate a visualization similar to that you can see in the attached image (Mu et al., 2012, Fish and Shellfish Immunology Journal).

15 September 2012 7,903 6 View

What should a smart water system look like?

Our team is designing a smart water C&C dashboard for a smart city initiative. As we look into the current smart water system landscape, we couldn't reconcile to a definitive solution that...

16 February 2021 1,094 3 View

How to disaggregate country data into city ones?

I'm using a number of indicators in my research which are not available at a sub-national level. Is there a reliable method for disaggregating the country data into city ones? (preferably with a...

16 February 2021 6,861 3 View

Master's thesis ideas related to City Marketing and City Branding?

I have started collaborating with a small city that is planning to attract international talents from the capital city. The city municipality still doesn't know what audiences can fit better with...

09 February 2021 2,081 3 View

Which are the factors that determine in a tourist the destination for their travel?

I'm working on a study of how selection criteria of Tourism have changed considering Pandemic + Vaccination expectation, so propose a new prediction behavioral dynamic model for Riviera Maya (Mexico).

09 February 2021 314 7 View

How can I label an antibody to determine how far it is travelling from the microdialysis probe?

I am trying to set up a new experiment where I am simultaneously utilizing reverse dialysis (antibody) through a microdialysis probe and a Miniscope for one-photon calcium imaging. Both pieces of...

09 February 2021 3,981 1 View

What questions should i include in my questionnaire?

I am writing my dissertation, about adults who trained at elite status in their youth, i am constructing a questionnaire with the goal of seeing, how many sustained injury, how many sustained...

28 January 2021 4,787 3 View

Is there a solution to this conundrum on freefall into a black hole?

18 January 2021 1,582 3 View

SEM for panel data ?

I'm trying to find the effect of smart cities on sustainable development. I collected data for several indicators of smartness of a city for 15 cities over 12 years. seems like panel data (GMM...

12 January 2021 9,914 5 View

Can Anything Pass From One Universe To Another?

Roger Penrose in the book Cycles Of Time requires all mass and energy to be converted to electromagnetic radiation. This is the sequential end of time in an old universe 3D not 4D and the setting...

02 January 2021 9,086 3 View

How to describe the method described below?

I do research on the relationships between universities and local governments by asking the research question (how) can universities contribute to the design (and implementation) of public...

29 December 2020 4,522 12 View