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Dear Misti Correct me if I m wrong, you are planing to isolate bacterial genomic DNA then will digest it with restriction enzyme (s) and finally will clone the fragments??? Am I right?
You can use:
Restriction enzymes from New England Biolabs or Fermentas
For plasmid isolation or purification of DNA Fermentas or Real Biotech Corporation kits
E. coli DH10B as chemically competent bacteria
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A fungal strain was treated with nanoparticles. We want to do an environmental SEM analysis. So could anyone share your views on preparing the sample? Thank you.
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