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Dear Misti Correct me if I m wrong, you are planing to isolate bacterial genomic DNA then will digest it with restriction enzyme (s) and finally will clone the fragments??? Am I right?
You can use:
Restriction enzymes from New England Biolabs or Fermentas
For plasmid isolation or purification of DNA Fermentas or Real Biotech Corporation kits
E. coli DH10B as chemically competent bacteria
Important factors to consider include: flexibility to obtain several outcome measures from the ECGs, ease of use and reasonable cost.
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