Saber Delpasand Khabbazi

3 Questions 42 Answers 0 Followers

Questions related from Saber Delpasand Khabbazi

Any recommendation for Bacmid DNA isolation?

Bac-to-Bac® Baculovirus Expression System (Invitrogen) I have cloned a gene of interest into pFASTbac plasmid and this construct has been used to generate recombinant bacmid DNA. Blue and white...

30 November 2020 5,600 6 View

Is it possible to have some parts of T- DNA broken while plant transformation?

I m trying to transfer a ~ 10 kb T-DNA (containing some gene fragments) to tobacco plant via Agrobacterium. While Screening the transgenic plants by PCR I face different results that marks the...

30 May 2016 2,763 11 View

How can we get rid of bacterial clump formation in Agrobacterium (GV2260) culture?

I have tried YEB, YEM, LB mediums but still facing the problem. temrature: 28c; rpm: tried 190 and 230; antibiotic rifampisin(tried 10, 25 and 50mg/l) and kanamycin(50mg/l) also I tried culture...

04 June 2015 2,255 6 View