Hello everyone, i am interested in studying the developmental process of a neuron across different days. To start with, I wanted to isolate cortical neurons from E16,E17 pups and culture them in a dish. My question is -DIV 0, DIV 1 neuronal age will be the same as neurons of post-natal day 1, post-natal day 2 pups. How do you do a comparative study so that the cells in the dish will mimic the same developing pattern as the post-natal pups? Thanks