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Questions related from Richard Jayaraj
Hello everyone, I would like to purchase a hypoxic chamber to study the effect of magnetic stimulation on hypoxic N2A cells. The cells will be exposed to hypoxia (1% oxygen) only for 3 hours...
21 September 2021 4,583 0 View
Hello Researchers, I am trying certain new antibodies for first time in our lab for cell culture experimental samples (N2A mouse neuroblastoma cells). Due to low protein concentration in cell...
07 September 2021 1,249 2 View
I would like to infuse rats with drugs of our interest. However, we also need to keep animal under sedation by administrating pentobarbitol via jugular vein with the help of syringe pump since we...
16 September 2020 7,846 5 View
Hello everyone, my interest is to understand the molecular mechanisms involved in anaphylactic shock using Wistar rats. While i inject non-allergic naive rats with iv bolus (1 mg ova in 1 ml),...
28 August 2020 9,866 2 View
Hello everyone, i am interested in studying the developmental process of a neuron across different days. To start with, I wanted to isolate cortical neurons from E16,E17 pups and culture them in a...
30 January 2020 1,814 1 View
I would like to quantify a desired protein from isolated exosomes (plasma/tissues). I was wondering if western blotting would be feasible ?
27 January 2018 7,540 3 View
I have been trying hard to find labs in India to learn MCAO but it ended in vain so it will really helpful if i can get an opportunity for a basic training. Thanks
22 June 2017 5,343 1 View
I am purchasing alpha synuclein commercially and it contains mostly oligomers and i need monomers for my experiments..
07 May 2015 3,653 3 View
Since the cathode buffer is blue, gel is stained with blue, I couldn't see clear protein bands after silver staining.
20 April 2015 8,778 2 View
I get very small peaks if I load 0.3 microgram (total concentration)
31 March 2015 6,239 7 View
