Richard Jayaraj

6 Questions 9 Answers 0 Followers

Questions related from Richard Jayaraj

What is the best hypoxic chamber- Biospherix or Coy Labs?

Hello everyone, I would like to purchase a hypoxic chamber to study the effect of magnetic stimulation on hypoxic N2A cells. The cells will be exposed to hypoxia (1% oxygen) only for 3 hours...

21 September 2021 4,529 0 View

Can we use mice brain tissue to standardize antibody concentration that will be later used for cell culture samples?

Hello Researchers, I am trying certain new antibodies for first time in our lab for cell culture experimental samples (N2A mouse neuroblastoma cells). Due to low protein concentration in cell...

07 September 2021 1,196 2 View

Can we infuse drug along with sedative?

I would like to infuse rats with drugs of our interest. However, we also need to keep animal under sedation by administrating pentobarbitol via jugular vein with the help of syringe pump since we...

16 September 2020 7,681 5 View

Ideal saline volume for intravenous injection in rats ?

Hello everyone, my interest is to understand the molecular mechanisms involved in anaphylactic shock using Wistar rats. While i inject non-allergic naive rats with iv bolus (1 mg ova in 1 ml),...

28 August 2020 9,684 2 View

Can we quantify a desired protein concentration from isolated exosomes through western blot?

I would like to quantify a desired protein from isolated exosomes (plasma/tissues). I was wondering if western blotting would be feasible ?

27 January 2018 7,398 3 View

Is the minimum concentration of protein to be loaded in FPLC sephacryl s-200 column?

I get very small peaks if I load 0.3 microgram (total concentration)

31 March 2015 6,191 7 View