Richard Jayaraj

11 Questions 19 Answers 0 Followers

Questions related from Richard Jayaraj

What is the best hypoxic chamber- Biospherix or Coy Labs?

Hello everyone, I would like to purchase a hypoxic chamber to study the effect of magnetic stimulation on hypoxic N2A cells. The cells will be exposed to hypoxia (1% oxygen) only for 3 hours...

21 September 2021 4,597 0 View

Can we use mice brain tissue to standardize antibody concentration that will be later used for cell culture samples?

Hello Researchers, I am trying certain new antibodies for first time in our lab for cell culture experimental samples (N2A mouse neuroblastoma cells). Due to low protein concentration in cell...

07 September 2021 1,272 2 View

Can we infuse drug along with sedative?

I would like to infuse rats with drugs of our interest. However, we also need to keep animal under sedation by administrating pentobarbitol via jugular vein with the help of syringe pump since we...

16 September 2020 7,866 5 View

Ideal saline volume for intravenous injection in rats ?

Hello everyone, my interest is to understand the molecular mechanisms involved in anaphylactic shock using Wistar rats. While i inject non-allergic naive rats with iv bolus (1 mg ova in 1 ml),...

28 August 2020 9,889 2 View

Question about cell culture age vs post-natal neuronal age?

Hello everyone, i am interested in studying the developmental process of a neuron across different days. To start with, I wanted to isolate cortical neurons from E16,E17 pups and culture them in a...

30 January 2020 1,821 1 View

Can we quantify a desired protein concentration from isolated exosomes through western blot?

I would like to quantify a desired protein from isolated exosomes (plasma/tissues). I was wondering if western blotting would be feasible ?

27 January 2018 7,551 3 View

Im very interested to learn Middle Cerebral Artery Occlusion in Rats and i'm willing to travel anywhere within India. Opportunity?Please let me know.

I have been trying hard to find labs in India to learn MCAO but it ended in vain so it will really helpful if i can get an opportunity for a basic training. Thanks

22 June 2017 5,349 1 View

How do I break down alpha-synucien oligomers into monomers?

I am purchasing alpha synuclein commercially and it contains mostly oligomers and i need monomers for my experiments..

07 May 2015 3,662 3 View

Could anyone suggest a way to get rid of blue color from the gels for silver staining from a NuPAGE gel?

Since the cathode buffer is blue, gel is stained with blue, I couldn't see clear protein bands after silver staining.

20 April 2015 8,785 2 View

I am trying to make alpha-synuclein monomers, oligomers and fibrils. Can anyone give me information about optimum agitation speed?

The concentration of alpha synuclein is 25-100 micromolar and i use 800 rpm, 37 degree celsius. I am concerned about the sample volume (30 microlitres in 1.5 ml tubes) and whether agitation helps...

10 April 2015 9,213 20 View

Is the minimum concentration of protein to be loaded in FPLC sephacryl s-200 column?

I get very small peaks if I load 0.3 microgram (total concentration)

31 March 2015 6,252 7 View