Quesion about the SMGT technique?

05 July 2019 1 1K Report

Please I want to ask you a quesion about the SMGT technique. Should I treate the vector with restriction enzyme for linearlization before sperm uptake experiment (incubation between sperm and linearlized vector) to increase the spem uptak?.

It doese not matter if I used a circular vector in the sperm incubation?

Best Regards

Shakweer

Zigomar da Silva

Dear Shakweer.

It is not necessary to linearize the plasmid to increase its sperm uptake. In fact, circular plasmids are more efficient at being internalized. However, linearization of plasmids could increase its genome integration.

You can read more in Oddi et al., (2012) (DOI: 10.1002 / mrd.22124)

Regards

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