Does anybody isolate and identify the organism that produce methane in rumen?

What are the key considerations for optimizing exosome isolation protocols from feces, ruminal fluid, and rumen chyme from cattle?

I have several inquiries regarding the isolation of exosomes from cattle: What are the most effective methods for isolating exosomes from feces, ruminal fluid, and rumen chyme? How do the...

02 April 2024 9,954 0 View

Which culture media is appropriate for methanogens?

I want to culture methanogens from the fecal sludge sample. Which culture media should I use?

14 March 2024 571 0 View

What causes high nitrogen production in an anaerobic digester?

Anaerobic digestion of rice straw and food waste designed for hydrogen production ended up producing more nitrogen, and less hydrogen. The inoculum is a seed sludge, (the source/details are...

01 December 2023 3,394 4 View

What are the white spots on the surface of the rumen+buffer mixture?

We are using the ANKOM RF gas production system to incubate feeds in a rumen + buffer solution and after 48 h white spots have started appearing on the surface. What would that be and how do we...

27 September 2023 4,793 0 View

Rumen Microbiota of Goat and Cattle?

What is the difference between the goat and cow microbiota? To my understanding and readings; goats tend to have a strong microbiota, therefore they can synthesize more ADF & and NDF. Please...

13 September 2023 2,909 3 View

How dose CO2 transport the membrane of methanogenic archaea?

Hi, everyone. May I ask if anyone knows the specific mechanism by which carbon dioxide (CO2) passes through the cell membrane of methanogenic archaea, is it similar to that of bacteria?

13 September 2023 9,548 0 View

Does PeiR peptidase have an active site?

PeiR is a lytic enzyme which is a methanogen virus that infects Methanobrevibacter ruminantium M1. Do all protease have active sites, if so how do I find it with the sequence only.

12 July 2023 8,548 4 View

Can you suggest any antifoam that in your experience does not interfere with methanogenesis?

I am doing ex-situ biomethanation and as I intensify my process I have encountered foaming. Have you had similar experience and if yes do you know of any antifoaming agents that does not affect...

18 June 2023 8,365 1 View

(new or) innovative technology in ruminant feed??

hi can anyone introduce a new technology or an article about ruminant feed, please? thank you.

05 May 2023 7,040 7 View

Dose anyone have the full text of Official Methods of Analysis of AOAC Internationa ??

Hi I need that for my dissertation, but I can't find a file about it. Can anyone guide me, please??

03 May 2023 9,719 2 View