Does anybody isolate and identify the organism that produce methane in rumen?

GC COLUMN SELECTION, Can Bpx70 column be used for volatile fatty acids in rumen fluid in aqueous state on GC-FID?

I want to analyze rumen fluid samples in aqueous state for volatile fatty acids on a GC-FID. We only have a Bpx70 column. Can this column be use?

24 September 2020 7,797 4 View

How to prepare Volatile fatty acids standard mixture, from individual acids standard for calibration/quantification of rumen VFA in unknown samples?

I want to prepare Volatile fatty acids standard mixture from individual acids bought from Sigma Aldrich. I have seen different volume mixture contradicting. I need a standard procedure/ volume...

17 September 2020 2,219 3 View

How to detect Methanogens using Flow Cytometry from wastewater?

Hi everyone! I'm trying to identify methanogens from wastewater using flow cytometry . Any idea on how to go about it? which stains or flurochromes we have to use? how do we go about isolation of...

24 June 2020 3,906 4 View

Is it possible to combine catalytic methanization with biological methanization to improve methane production?

With a catalytic pretreatment of the CO2 and H2 mixture to activate the reaction, then a second biological process using methanogenic bacteria, could the efficiency of the process be improved and...

04 April 2020 5,936 1 View

How to observe methanogens by using the fluorescence microscope ?

I want to know the selection of optical wavelength and other points to note in the process ,thanks.

15 April 2019 4,784 1 View

How to observe methanogens by using the fluorescence microscope ?

I want to know the selection of optical wavelength and other points to note in the process ,thanks.

03 April 2019 8,200 1 View

How can examine rumen to detect presences of anaerobic fungi?

Direct examination of rumen fungi

02 March 2019 7,835 2 View

Isolation and identification of proteins?

I am looking for the protein expression of Methanosarcina mazeii that can get expressed in the presence of acetate. I am not much aware of steps. I was planning to do cell-lysis and then SDS-PAGE...

20 February 2019 8,329 11 View

Anaerobic serum bottles pressure range?

Does anyone know the range of pressure that 120ml anaerobic serum bottles can tolerate (10-100 psi)?

01 February 2019 2,764 3 View

Isolation and identification of proteins?

I am looking for the protein expression of Methanosarcina mazeii that can get expressed in the presence of acetate. I am not much aware of steps. I was planning to do cell-lysis and then SDS-PAGE...

01 February 2019 6,089 12 View