Regarding cloning of Nus His tag fusion construct, to express double tagged fusion protein ?

Strategy 1) I have prepared N- Nus and C- His(6-His) tagged fusion construct of my protein of interest in pET43.1a by using SpeI and XhoI restriction enzyme and Prescission protease recognition...

09 October 2017 2,905 3 View

Sonication of protein sample?

For the expression of subtle Nus tag fusion protein I have optimized sonication for 5ml culture, 50 percent amplitude, 1pulse off 1pulse ON for 30 second. With repeated cycles with the gap of...

05 June 2017 8,975 10 View

How to calculate bacterial cells /ml from OD600 in Rosetta cells?

I want to calculate total number of Rosetta cells by using OD 600 , please explain.

04 May 2017 1,598 7 View

How much concentration of Protease inhibitor cocktail should I use in buffer?

How to decide how much concentration of protease inhibitor cocktail is required in buffer for protein expression ?

04 May 2017 6,702 3 View

150 mg/ml Rifampicin preparation in methanol ?

02 March 2017 8,268 5 View

Regarding linear and exponential PCR amplification during quick change and Inverse PCR site directed mutagenesis (SDM)?

10 November 2016 5,582 2 View

How to select molar ratio for cloning ?

I am interested to know whether a 1:3 molar ratio is ok with a 5.4 kb vector [5.4 kb after double digestion,(actual size of vector is 7.2 kb) ] and 600 bp insert DNA ? Is there any relation for...

09 October 2016 2,707 5 View

Regarding polynucleotide kinase (PNK) phosphorylation and heat inactivation after ligation of site directed mutated DNA ?

I am using NEB mutagenesis protocol (not kit) for point mutation, primers are designed back to back orientation and after PCR I want to perform PNK phosphorylation. I have thermoscientific PNK...

09 October 2016 1,545 1 View

Interpretation of gel before and after DpnI digestion?

09 October 2016 6,722 6 View

Not getting desired length of PCR product with Q5 polymerase ?

I amplified human gene cDNA(600 bp) with Q5 pol but after amplification I got 500 bp band with some nonspecific bands of less than 500 bp, then I performed gradient PCR and again I got 500 bp band...

09 October 2016 8,405 7 View

error in running a model in Abaqus

Excessive rotation of nodes in node set ErrNodeExcessRotation-Step1

17 August 2021 0 0 View

error in modelling model in Abaqus

Excessive rotation of nodes in node set ErrNodeExcessRotation-Step1

17 August 2021 0 0 View

MD MOE RMSD

How to calculate the RMSD values for a MD simulation using MOE?

07 August 2021 0 0 View

MD MOE RMSD

How to calculate the RMSD values for a MD simulation using MOE?

07 August 2021 0 0 View

MGL tools

20 June 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

what is the simplest definition of ion exchange capacity

What this unit identifies for the ion exchange capacity ca. 0.92 meq. g-1. I am a newcomer in the membrane area. I just started. I highly appreciate your help

08 May 2021 0 0 View