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Why I am getting a red dot( or false satining)when I stain my primary cultures only with DAPI?This spots looks red in DAPI filter and looks green in FITC filter and red in TRITC filter irrespective of the fact that I am not staining it with FITC or TRITC. .The red dots are not in my nuclear region but looks to be in surrounding region .It appears as false positive.This problem is only with tissues and primary cell culture staining.Secondary cultures do not give such false staining.

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