I am working on biofilms for clinical isolates of pseudomonas. What is the best media for growing overnight cultures of pseudomonas as we as biofilms?
2. How do we prepare M63 minimal media (supplemented with mgso4, glucose, casamino acid)
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Do a simple 2 mins google search and you will find plenty of protocols how to prepare M63 minimal media, here is just one of them
https://www.protocolsonline.com/molecular-biology/media-molecular-biology/m63-medium/
Why do you not try with LB media, try it. You can also use M9 minimal media.
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