Samples to study circular dichroism (chirality)?

I want to build a low-cost polarimeter to study circular dichroism (chirality). What type of samples can I use? I am looking for samples that can be found outside the laboratory.

01 March 2021 9,101 3 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

RIA. How to measure?

When you use RIA, with a control the unknown sample with antibodies, you bare in mind the binding sites of the antibodies. However you still need to measure labelled antigen (radioactive) and not...

28 February 2021 2,133 3 View

Benefits of the 24 welled plate?

We have always used the 96 welled plate, the 48 welled plate but apparently there is much evidence on the 24 welled plate and above all its benefits.It states it is the same you would use on ELISA...

28 February 2021 1,149 1 View

Algorithms to filter corrective max and min from a set of data?

I've got this 5 year Direct Normal Irradiance data I downloaded from https://re.jrc.ec.europa.eu/. I ordered this by year and by every 24 hours because I need the DNI curve by max in summer, min...

17 February 2021 2,777 5 View

Flexibility of RNAseq?

When you use RNAseq I understand that it must follow certain conditions when you use it. One of them is the insertion of a polyA tail. The insertion of the polyA must include then...

05 February 2021 3,770 4 View

Is there an open source tool the measures the GRAVY index of a peptide incorporating the PTMs and unusual amino acids (e.g. hydroxyproline)?

Right now, I am using ProtParam to compute GRAVY index of peptides. However, some of my peptides are post-translationally modified, and contains unusual amino acids. I assume that ProtParam does...

29 January 2021 8,300 4 View

What type of spectral radiation has greater penetration on materials, UV or IR? What book or source do you recommend for information on this?

I need information concerning the penetration and reflection capacity of ultraviolet and infrared radiation wavelengths on different most common materials. Edit. Can somebody recommend a book to...

16 January 2021 8,051 6 View

Could be estimate or simulated the electrical properties of thin films obtained from new organo-metallic compounds?

In the context of synthesized organo-metallic compounds, I am interested in the formation of thin-films over silicon wafers. Among another characteristics to be investigated, electric properties...

13 January 2021 7,575 2 View

How long does the peptide cleavage cocktail last before it gets expired?

The cleavage cocktail is composed of Phenol/thioanisole/water/TFA/triisopropylsilane. I only prepare 10 mL cocktail per peptide but can the activity of the cocktail prepared at 100 mL last for a week?

10 January 2021 7,424 3 View

Can the potential of LIPIDMICS improve the diagnosis and understanding of metabolic changes in various neurodegenerative disease (PD, HD, AD, MS, etc?

08 November 2020 7,209 1 View

Are drugs that are susceptible to first pass metabolism readily excreted?

first pass metabolism decreases bioavailability and can increase elimination but does it increase excretion of susceptible drugs

22 July 2020 9,313 3 View

For what reasons the activity of soil microorganisms is higher in a newly dune-covered pine forest than where pines have been growing for a long time?

Maybe you could suggest articles on this topic. I researched the metabolic activity of microorganisms in mineral soils in Coronian Spit dunes.

09 June 2020 7,618 3 View

Why is COD removal more in biological anaerobic treatment?

Answer related to metabolism of bacteria would be appreciated.

23 May 2020 4,050 2 View

I need to buy as respirometer for measuring the metabolic rate of insects. I want to know to whom I have to contact?

I want to know the cost and company which it supplies

17 April 2020 2,079 3 View

Can you recommend excellent article(s) on metabolism?

Trying to make the most of my time staying at home in this pandemic. I will love you help with articles that give good overview on metabolism and metabolic diseases

20 March 2020 6,673 3 View

Which are the most effective ways to promote oxidative metabolism during cell culture?

I am looking for chemicals that can be used in mammalian cell culture to enhance oxidative metabolism (TCA cycle). Can NAD+ precursors (NR, for example) be used?

17 March 2020 7,378 4 View

What's the best way to image cancer tumor growth other than FDG?

Was quantifying clinical chemotherapeutic efficacy with FDG (radioactive glucose). This general read of metabolism a good match for highly metabolically active cancer cells. However, some...

18 February 2020 6,845 1 View

Can an autotroph run on and store ATP without carbohydrate storage?

what is mass-based and volume-based energy storage capacity of ATP and typical carbohydrates? Is it possible for an autotroph to be evolved/found that convert sunlight into energy of ATP, store...

10 November 2019 9,620 2 View

How much Protease inhibitor do I need to add to my mouse serum sample?

I am using Protease Inhibitor Cocktails (VWR_M221-1ML). I reconstituted the inhibitor, but I am not sure how much of the protease inhibitor do I add to my sample. I am preparing the sample for a...

09 October 2019 7,346 3 View