In contrast to the system's RT Quantitative PCR experiment type, which uses threshold cycle (Ct) values to calculate initial quantities, Quantitative Plate Read experiments use only endpoint fluorescent value for calculating the initial quantities in Unknowns wells. Both of them use standard curve to estimate the amount of target present. But I don't know to choice between these two types of experiments, in terms of advantages and disadvantages. I do know that in real time PCR the fluorescent lecture take place at each cycle and in the other experiment the fluorescent lecture is made at the beginning or/and the end of the essay.