Quantitative PCR versus Quantitative Plate Read? When to use one or another?

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Which air pollution/air quality books do you recommend for working with environmental engineering students?

I am exploring up-to-date books on air pollution and air quality, and I would like to know which books do you recommend for environmental engineering students.

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Numerical taxonomy with SPSS?

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What are the best paid maths journals?

Do they accept everything or they still pass them on a sieve?

12 January 2021 3,515 15 View

How do I delete a publication that I added?

I added two times the same thing by mistake and I need to remove one. Thanks.

11 January 2021 6,118 5 View

PScad compatibility with SIMULINK?

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06 January 2021 8,226 1 View

Journals with low APC or no APC?

Do you know any journals in the area of medicine/pediatrics/nutrition that publish protocols of systematic reviews? No APC or Low APC

04 January 2021 292 14 View

I would like. How they can be simulated the deformational processes of valvular aortic stents??

We have a projet about TAVI

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Why is Academia.edu so bad?

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Any suggestion for counting a large amount of small seeds?

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Large Sample Size and Differences in Chi-Square Statistic?

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What is your perception on using visual analysis or quantitative approaches to evaluate data from single-case research designs (n of 1 trials)?

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03 March 2021 687 1 View

How do I increase the yield of my PCR product?

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Waters 2707 HPLC autosampler problem?

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How to quantitative analize the data of TNBS assay for gelatin?

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Should we change media after1st 6hrs after plating HUVEC bought from INVITROGEN?it's not recomended by invitrogen.Will DMSO present in stock matter?

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How to normalise data on OPM package from R Studio ?

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Sample dilution for quantitative analysis using ELISA?

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Do you know good and user-friendly programs to measure plant quantitative traits?

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Does anyone know HRM channel in a 2 plex QIAGEN Rotor- Gene Q qPCR can be used as a separate channel?

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01 March 2021 8,544 1 View

How to address this issue in the analysis of Real-Time PCR results?

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Oscar Hernandez

In contrast to the system's RT Quantitative PCR experiment type, which uses threshold cycle (Ct) values to calculate initial quantities, Quantitative Plate Read experiments use only endpoint fluorescent value for calculating the initial quantities in Unknowns wells. Both of them use standard curve to estimate the amount of target present. But I don't know to choice between these two types of experiments, in terms of advantages and disadvantages. I do know that in real time PCR the fluorescent lecture take place at each cycle and in the other experiment the fluorescent lecture is made at the beginning or/and the end of the essay.