By which mechanism does potassium hyrdoxide works as an activator in the preparation of activated carbon?

Activated carbon is prepared from biomass using KOH as the activating agent. What is the possible reaction between KOH and the biomass?

14 February 2021 8,095 4 View

Should studies published in predatory journals be included in systematc reviews?

I am conducting a systematic review of intervention, and i observed that one included study could determine the statistical significance of the final meta-analysis. Problem is, that article has...

13 April 2020 922 9 View

OCR system for phonetic (IPA) symbols?

I need to convert manually annotated IPA transcriptions into digital format. Does anybody know of an OCR system able to recognise IPA symbols (even just for English phonemes)?

25 March 2020 5,982 0 View

Is it possible to read the deformation field file produced by SPM during normalization?

If (x,y,z) are the voxel coordinates of a point of the brain scan before normalization, what is its position after applying the deformation field generated during the normalization?

19 February 2020 10,004 3 View

Bibliography on the effect of soils on nectar and sap?

10 September 2019 8,922 4 View

What is the correct phenol red permeability through Caco2 monolayer?

Dear all, I am performing Caco2 permeability experiments using transwell inserts and I am using phenol red permeability assay to verify the monolayer integrity. What is the maximum phenol red...

06 March 2019 6,726 6 View

How much solvent (ethanol) would I need to extract capsaicin (and other capsaicinoids) from siling haba?

Siling haba is a variation of Capsicum annuum var longum. The capsaicin extracted will be tested against aphids to test its insecticidal properties. Someone suggested that I should use 5ml/g or 2g...

12 February 2019 1,373 3 View

How much ethanol do I need in extracting capsaicin from red bell peppers?

The capsaicin recovered will be tested against aphids.

04 February 2019 5,811 5 View

Is anyone aware of studies concerning geostatisics in the environmental field?

I'm looking for studies on environmental analysis carried out by geostatistics

16 January 2019 5,356 4 View

CVD adhesion on silicon substrates?

Dear all, I am currently trying to uniformly deposit organic semiconductor via thermal CVD at low pressure (around 10 Torr), but I sometimes get either no deposition or not homogeneous deposition...

21 October 2018 3,185 4 View

Can linear DNA be used as template for site-directed mutagenesis?

Is it possible to induce site-directed substitution mutation by quick-change method on linear dsDNA? or it has to be cloned in some vector? If yes, should it be treated with the Dpn1 enzyme...

03 March 2021 401 4 View

New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before?

Question to you and THEM, the New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before? There appears to be a core problem for...

02 March 2021 3,024 2 View

Does anyone have a recommendation for yeast reporter gene plasmid/protocol?

Hi, could anyone recommend a plasmid and/or protocol for reporter gene assay in S. cerevisiae? I want to assess the effect of growth conditions on a transcription factor, so I want to clone it`s...

01 March 2021 210 1 View

Does anyone know HRM channel in a 2 plex QIAGEN Rotor- Gene Q qPCR can be used as a separate channel?

I am going to have 3 different probes in my qPCR work that I am going to do. But I realized that the machine we have in the lab is a Rotor-Gene Q 2plex HRM Platform, saying it has green, yellow,...

01 March 2021 8,544 1 View

Expression cloning by using cDNA,do I need to modify the cDNA first?

I am going to have a expression cloning of mammalian gene by using shuttle plasmid to transforming the E.coli However I don't know I should only inserting the Coding sequence ,or I can...

28 February 2021 5,440 3 View

Unknown debris sheets observed in cell culture flasks?

Hi, We culture neural progenitor cells in T175 culture flasks coated with PLO-Laminin. We have successfully grown these cells with our protocol for months. Recently, we observed large sheets...

25 February 2021 3,443 4 View

Difficulty in cloning of 1kb gene into vector?

I am facing difficulties in cloning a 1kb gene into a vector (pJIT163). I have my gene on interest (GOI) in pUC57 and want to clone in pJIT163 using SalI and BamHI restriction sites. I am getting...

25 February 2021 3,221 7 View

Can we recognize signs of the "volatility clustering" property of a time series in its time domain plot?

My purpose is to recognize the volatility clustering property without statistical tests such as The Lagrange Multiplier (LM) test.

24 February 2021 4,150 3 View

How to run repeated measures in mvabund?

I have a dataset of fish species abundances collected from 6 different habitats over the course of 30 years. Fish were collected multiple times per year and environmental variables were recorded...

21 February 2021 3,838 1 View

Is there a method for evaluating nanoparticle adhesion to epithelial tissue in vitro?

I want to measure how well nanoparticles can transfect cells based on NP adhesion properties. In the literature I find people do it in animal studies, and epithelial adhesion helps in transfection...

21 February 2021 7,791 3 View