Is there any way to calibrate transducer without needle hydrophone?

Is there any way to calibrate transducer without needle hydrophone? Also any one know low cost hydrophone as precision acoustic is quite costly.

16 December 2020 1,070 2 View

How to convert mV output into MPa?

I am working on acoustic characterization of microbubbles using two transducers arranged at 90 degrees. During calibration, I am receiving response in dBm or mV. How I can know the PNP of my...

14 December 2020 1,814 5 View

What is the dielectric constant(Real and Imaginary parts ) of MASnI3 for 300 to 1200nm wavelength?

What is the dielectric constant(Real and Imaginary parts ) of MASnI3 for 300 to 1200nm wavelength? as I need it for Perovskite solar model design in Comsol Multiphysics

25 November 2020 1,350 1 View

Is there any way to prepare Polymeric Nanoparticles without adding water??

I am making Poly Glycerol Sebacate nanoparticles by solvent evaporation and nanoprecipitation, but material got hydrolyzed upon adding water. I want to replace aqueous phase. Which chemical should...

21 October 2020 8,319 9 View

Path to node for column diameter while linking Aspen with excel or Matlab?

i am optimizing a reactive distillation column. I have simulated a base case in Aspen Plus and now want to calculate its total annual cost in Matlab. while linking aspen Plus and Matlab i need...

01 October 2020 207 2 View

What happen if sebacic acid and glycerol were reacted for hour and then left the continued next day??

I have to end a reaction in process due to sone emergency. I was synthesizing poly glycerol sebacate. I reacted them at 170 under nitrogen for an hr and then stopped. Normal duration is 4 hr....

23 September 2020 7,827 5 View

Instead of gold or carbon coating, may I use platinum sputtering to get SEM image of a non-conducting material?

I actually don't have gold and carbon. I would like to know if I can do platinum sputtering on my sample to get SEM image? My sample is a non-conducting nanofiber

22 September 2020 2,470 3 View

How to check previous TC track record from the Japan Meteorological Agency (JMA)?

The TC track record can be checked easily from the Shenzhen Typhoon website, but how to check such records from the JMA website? Looking forward to a positive response.

31 August 2020 6,285 3 View

How to prevent hydrolysis of Pre-Polymer Poly Glycerol Sebacate?

I am working on encapsulating drug in Pre-PGS by solvent evaporation method. In which organic phase is PGS dissolved in THF, drug in water with tween 20. I am getting little particles at end. But...

02 April 2020 497 11 View

How to find Mean pixel intensity of ROI by using Image Processing Tool in MATLAB?

I am trying to find mean & SD of selected ROI via image processing tool. It is showing individual pixel intensity but not mean. Did I have to write a code for it?

25 February 2020 6,225 3 View

Why does blocking with my immunogenic peptide cause nuclear fluorescence in ICC?

Hi all, I am validating a polyclonal antibody raised against human ANT4, through WB and ICC. In most of my controls, the antibody shows very little off-target binding, but when I pre-incubate the...

03 March 2021 5,999 3 View

Does Napabucasin soluble in dichloromethane and in ethanol?

I need to prepare a solution of Napabucasin (inhibitor of cancer cell stemness) in dichloromethane. The drug is expensive, so maybe somebody already tried to do it. If it is soluble, what is the...

02 March 2021 9,945 3 View

Help request in CST microwave?

im working now on TPV solar cells and metamaterials and i realy need our help in CST microwave i have a tpv cell simulation and i want to extract particullarly the absorbtion of only the active...

02 March 2021 2,246 2 View

Tooth extraction and subsequent dental implant placement in Sprague-Dawley rats induce differential changes in anterior digastric myofibre size and ?

01 March 2021 7,254 3 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View

Dapi fluorescence protocol for colon cell lines ?

28 February 2021 2,534 2 View

Does RNAiMAX transfection reagent work in serum free medium?

I diluted siRNA and RNAiMAX in opti-MEM and added to the cells which they were in the growth medium. Is it a right way? or should I culture cells in the opti-MEM medium for a while and not in...

26 February 2021 10,041 3 View

Has anyone had luck using cell lines other than HEK 293 (such as COS1) to package lentivirus?

I am looking to package lentivirus in endothelial cells but I am opposed to using 293 cells due to personal ethics regarding the source. I have done some reading and saw that COS1, COS7, & CHO...

25 February 2021 3,030 1 View

Unknown debris sheets observed in cell culture flasks?

Hi, We culture neural progenitor cells in T175 culture flasks coated with PLO-Laminin. We have successfully grown these cells with our protocol for months. Recently, we observed large sheets...

25 February 2021 3,443 4 View

How do I transfect a large plasmid into PC12 cells with nerve growth factor (NGF) receptor?

I have a large 16 kb plasmid, which I need to transfect into PC12 cells. Lipofectamin 2000 didn't work and with GFP alone the transfection rate is very low. I also tried the neon invitrogen...

25 February 2021 6,635 5 View