I would like to quantify DNA using gel photos instead of extracting the DNA from the gel. Please help if you have information on a software that I can use to do this.
We normally do this with Adobe Photoshop. When you open it, a "histogram" appears on the upper right side. When you mark your region of interest, it gives you a number corresponding to the "white-intensitiy".
Hi Elizabeth
You can use any of the software on the 'geldoc' system you might be using for caturing your gel photographs....if not you can still use many 1-D gel analysis software freely available on internet....'Labimage' is also a simple a good software...
Check the ImageJ software (freeware).
Be sure to take picture with allways the same intensity and take care to avoid saturation problems.
To know your quantity, do a calibration curve by using the given quantities of DNA for ladder bands.
you can use the geldoc software or still analyse the image with imagej .....
or you can do virtual gel by using Bioanalizer but this is quite expensive method but in the other hand quick and precise
now dear caliper imaging system and MultiNA technologies are also available where u can quantify the DNA in less cost then bioanalizer...but still they r little expensive, as per Arnab suggestion...u can go for quantification by software....if you use AlphaInotech corp ..FluorChem 5500 software...u can easily quantify with rf values
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