13 Questions 10 Answers 0 Followers
Questions related from Elizabeth Siago Kusia
I am trying to rear saturniid moths in the lab and they keep diapausing at the pupal stage. They stick at this level for months on and am unable to raise a colony. Anyone knows how I can break the...
10 October 2017 3,443 3 View
We are trying to maintain a colony of saturniid moths collected from the field. However, we are experiencing problems with mating. The males and females emerge at different times. These moths only...
11 November 2016 4,380 4 View
I want to quantify RNA plant viruses using RT-qPCR. I have some primers that I found in a publication. The primers were used witha taqman probe. However, I have SYBR green mastermix in the lab. Is...
08 August 2015 8,907 5 View
I am looking for alternative hosts of a maize virus. I want to sterilize the leaf samples of the suspected hosts before total RNA extraction, since I am working with an RNA virus. I just want to...
10 October 2014 1,669 3 View
I tried to submit sequences to the GenBank, but got an error message indicating that there are non-IUPAC residues in the sequence. What does this mean and what can I do?
09 September 2014 6,896 3 View
Am working on a plant RNA virus. I did RT-PCR then purified the PCR products and sent them for sequencing. Note: I did not excise gel. However am not sure whethet the sequences I got are ok. The...
08 August 2014 830 5 View
I want to collect leaves from maize and wild grasses from the field but am not sure what method will be best to maintain the integrity of the viral RNA for extraction and RT-PCR. Should I use FTA...
01 January 2014 2,071 3 View
I am working with a plant pathogen that cannot be cultured, I want to determine the difference in concentration of the pathogen in different parts of the plant for the purpose of standardizing my...
07 July 2012 2,594 8 View
I would like to quantify DNA using gel photos instead of extracting the DNA from the gel. Please help if you have information on a software that I can use to do this.
03 March 2012 6,919 9 View
I am using LAMP to amplify 16SrRNA for detection of phytoplasma in napier grass. In the past week, the negative control has been appearing positive on agarose gel electrophoresis. I even tried...
03 March 2012 498 3 View
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11 November 2011 9,788 38 View
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10 October 2011 3,810 2 View
Hey guys, please explain to me how i would know that a buffer i have prepared is actually working e.g. ctab, TAE or TBE buffer. am from college and just secured a place as a molecular biology lab...
09 September 2011 2,646 2 View
