Dear Sir. Concerning your issue about the quantification of Chlorophylls with a Microplate Reader UV spectrophotometer. The procedure enables direct translation of any published equation developed on a 1-cm pathlength spectrophotometer to any format microplate with any sample volume. Chlorophylls were extracted with methanol because it is compatible with

cheap polystyrene plates; however, the principles apply to any solvent system. Keys to obtaining accurate and precise results include determining pathlength in the same solvent

used to extract chlorophylls, and avoiding evaporation. After taking these precautions,

there was a strong linear 1:1 relationship (R2 = 0.98–0.99) between microplate and

spectrophotometer estimates of chlorophyll concentrations. I think the following below link may help you in your analysis:

https://www.biotek.de/assets/tech_resources/Rapid%20Measurement%20of%20Chlorophylls%20with%20a%20Microplate%20Reader.pdf

Thanks

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Thank you so much. Does it also work with solvent mixtures? Ethanol was used at 80%