QTL/GWA studies with Drosophila Genetic Reference Panel (DGRP) lines?

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Is it okay to incubate ELISA plates (after adding biotinylated tracer) at 4C for two nights or more?

The protocol for sandwich ELISA kit that I am using requires me to incubate the plate at 4 degree Celsius overnight after adding the biotinylated tracer. Provided there is no microbial growth...

31 December 2017 5,160 5 View

Anaconda versus Enthought Canopy?

For simulations of some nonlinear systems in python, what are the pros and cons of Anaconda and Enthought Canopy, and also the IDE best suited (if any) for either of them. Based on my minimal...

07 August 2016 8,898 4 View

In a network of diffusive coupled oscillators how does coupling strength change as a function of concentration of coupling factor/agent?

In a network of coupled oscillators (diffusive coupling) while it is intuitive to expect that the magnitude of coupling increases with increase in concentration of coupling factor (provided the...

10 November 2015 3,404 8 View

How to avoid Drosophila isofemale lines from crashing?

I need to generate isofemale lines of Drosophila melanogaster but the last time we initiated a set of such lines, most of the lines crashed possibly due to inbreeding depression. I am aware that...

10 November 2012 5,062 5 View

What is the average efficiency of PCR reactions in general? (FYI: I use qPCR with sybr green chemistry in ABI Step One plus machine)

Ideally the efficiency of a PCR should be 100% and qPCR tutorials online say that it should be somewhere between 90% to 100 %. In one of the recent talks that I attended, I was told that if my PCR...

10 November 2012 3,699 16 View

How to learn more about SPSS and its Application?

I would like to learn more about SPSS and Its application especially in regards to data analysis. Please suggest me how I can learn more about it. Thank you so much.

11 August 2024 9,101 4 View

Can I base on reverse DNA sequences to perform alignment, convert to amino acids and GenBank submission?

I have reverse sequences (AB1 format), can I base on reverse DNA sequences to perform nucleotide alignment, convert nucleotides to amino acids and deposit the sequence in GenBank database?

11 August 2024 5,138 1 View

Baseline drift in HPLC? What causes this?

Hello, Why do i see this baseline drift when i compare my blank (black) to the sample (blue)? Any suggestions as to why this happened? Thank you!

11 August 2024 3,770 4 View

Text-Communication from the M1 Hand Area using BCI—and then there is Elon Musk?

Willett, Shenoy et al. (2021) have developed a brain computer interface (BCI) that used neural signal collected from the hand area of the motor cortex (area M1) of a paralyzed patient. The...

10 August 2024 7,180 0 View

Has anyone applied Python in the field of textile engineering for data analysis, automation, or smart textiles?

I'm currently exploring the application of Python in textile engineering, specifically in areas like data analysis, process automation, and the development of smart textiles. I'm interested in...

10 August 2024 7,429 2 View

How can I use the cif data obtained from rietveld refinement extracted via gsas2, for microstructural analysis using ETEX software?

09 August 2024 7,718 0 View

How are iso-frequency contours plotted?

Let's say we have a standard, regular hexagonal honeycomb with a 3-arm primitive unit cell (something like the figure attached; the figure is only representative and not drawn to scale). The...

07 August 2024 1,937 1 View

How to prepare the nanoparticle treated fungal sample for Environmental SEM analysis?

A fungal strain was treated with nanoparticles. We want to do an environmental SEM analysis. So could anyone share your views on preparing the sample? Thank you.

07 August 2024 5,307 1 View

How to normalize and take the significance of the MTT OD values with 3 replicates for the same cell-line?

Hi, I have a question about normalizing the MTT OD values for doing the statistical analysis. So, if we have 3 different plates and we call them 3 different replicates, so, first we would...

07 August 2024 8,106 4 View

Why does my protein refolded to beta sheet during thermal denaturation analysis?

Hi! So i attempted to understand a novel protein behavior towards heat application by analyzing its secondary structure change. I subjected the protein to a thermal denaturation analysis using...

06 August 2024 1,989 3 View