QTL/GWA studies with Drosophila Genetic Reference Panel (DGRP) lines?

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Is it okay to incubate ELISA plates (after adding biotinylated tracer) at 4C for two nights or more?

The protocol for sandwich ELISA kit that I am using requires me to incubate the plate at 4 degree Celsius overnight after adding the biotinylated tracer. Provided there is no microbial growth...

31 December 2017 5,068 5 View

Anaconda versus Enthought Canopy?

For simulations of some nonlinear systems in python, what are the pros and cons of Anaconda and Enthought Canopy, and also the IDE best suited (if any) for either of them. Based on my minimal...

07 August 2016 8,606 4 View

In a network of diffusive coupled oscillators how does coupling strength change as a function of concentration of coupling factor/agent?

In a network of coupled oscillators (diffusive coupling) while it is intuitive to expect that the magnitude of coupling increases with increase in concentration of coupling factor (provided the...

10 November 2015 3,286 8 View

How to avoid Drosophila isofemale lines from crashing?

I need to generate isofemale lines of Drosophila melanogaster but the last time we initiated a set of such lines, most of the lines crashed possibly due to inbreeding depression. I am aware that...

10 November 2012 4,971 5 View

What is the average efficiency of PCR reactions in general? (FYI: I use qPCR with sybr green chemistry in ABI Step One plus machine)

Ideally the efficiency of a PCR should be 100% and qPCR tutorials online say that it should be somewhere between 90% to 100 %. In one of the recent talks that I attended, I was told that if my PCR...

10 November 2012 3,537 16 View

How to compare two groups with only two measurements?

Hiiiii everyone! I have an enquiry on statistical analysis. I was looking for many forum and it's still cannot solve my problem. I want to compare means of two groups of data but only with two...

03 March 2021 8,796 3 View

Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence?

I am on the lookout for the Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence. Does anyone know where I can find it? Thank you in advance

03 March 2021 3,568 1 View

What kind of fluid could I use for a pitfall trap that also does not invalidate molecular testing?

Hi, I want to start testing pitfall trap to obtain ants samples, but I need to conduct molecular analysis on those insects. So, what kind of fluid can I use? Ethanol expires too early and I need...

03 March 2021 5,978 5 View

What is your perception on using visual analysis or quantitative approaches to evaluate data from single-case research designs (n of 1 trials)?

Results of single-case research designs (i.e., n-of-1 trials) are often evaluated by visually inspecting the time-series graph and computing quantitative indices. A question our research team is...

03 March 2021 687 1 View

What's the best way to measure growth rates in House sparrow chicks from day 2 to day 10?

What's the best way to measure growth rates in House sparrow chicks from day 2 to day 10? Since, the growth curve from day 2 to 10 won't be like the "Logistic curve" it might not follow logistic...

03 March 2021 1,401 3 View

Do we need to write protocol for a second study of a systematic review that has been registered and published?

I have conducted and published a systematic review and meta-analysis research with the topic related to public health and health pomotion (protocol was registed in PROSPERO). Now we want to...

03 March 2021 8,920 3 View

How to increase a model accuracy ?

dear community, my model is based feature extraction from non stationary signals using discrete Wavelet Transform and then using statistical features then machine learning classifiers in order to...

03 March 2021 6,994 5 View

Do I need to do linear regression before running mediation analysis using PROCESS MACRO by Hayes ?

I just wanted to check if I need to run a linear regression separately if I am using PROCESS MACRO to run mediation analysis. Thank you.

02 March 2021 4,359 3 View

How to quantitative analize the data of TNBS assay for gelatin?

Estemeed colleagues, I found some issues regarding the quantification of the data for TNBS assay. There are different protocols on how to perform that but it is clear to me the "fil rouge" that...

02 March 2021 2,616 1 View

Sample dilution for quantitative analysis using ELISA?

If the detection range is in ng/ml but the reference range is in ug/ml for a molecule or protein in serum or plasma .how to dilute and what is the initial volume to be taken for quantitative analysis

02 March 2021 7,670 3 View