KL Nikhil

6 Questions 21 Answers 0 Followers

Questions related from KL Nikhil

Is it okay to incubate ELISA plates (after adding biotinylated tracer) at 4C for two nights or more?

The protocol for sandwich ELISA kit that I am using requires me to incubate the plate at 4 degree Celsius overnight after adding the biotinylated tracer. Provided there is no microbial growth...

01 January 2018 5,131 5 View

Anaconda versus Enthought Canopy?

For simulations of some nonlinear systems in python, what are the pros and cons of Anaconda and Enthought Canopy, and also the IDE best suited (if any) for either of them. Based on my minimal...

08 August 2016 8,841 4 View

In a network of diffusive coupled oscillators how does coupling strength change as a function of concentration of coupling factor/agent?

In a network of coupled oscillators (diffusive coupling) while it is intuitive to expect that the magnitude of coupling increases with increase in concentration of coupling factor (provided the...

11 November 2015 3,366 8 View

QTL/GWA studies with Drosophila Genetic Reference Panel (DGRP) lines?

Has anyone here used DGRP lines for QTL mapping/GWA studies?

01 January 2014 3,977 2 View

What is the average efficiency of PCR reactions in general? (FYI: I use qPCR with sybr green chemistry in ABI Step One plus machine)

Ideally the efficiency of a PCR should be 100% and qPCR tutorials online say that it should be somewhere between 90% to 100 %. In one of the recent talks that I attended, I was told that if my PCR...

11 November 2012 3,647 16 View

How to avoid Drosophila isofemale lines from crashing?

I need to generate isofemale lines of Drosophila melanogaster but the last time we initiated a set of such lines, most of the lines crashed possibly due to inbreeding depression. I am aware that...

11 November 2012 5,032 5 View