Hi everyone,

I have to do qPCR to quantify microorganisms with the nirS gene but I have a problem with the standard. Indeed, due to a material problem I lost all my competent cells containing the plasmid with my standard nirS gene.

I was wondering what was the best solution in the emergency: Use an externe enterprise to synthesize an oligo corresponding to the sequence of the gene, order the reference bacterium for this gene and do the extraction and recovery of the gene...? Thank you for your help