Ct of target gene in Untreated condition is undetermined, how does performing Livak's method (DDCt method to generate fold change values (2–DDCT)?
You have mentioned as a co-author of one publication, but it is not exactly a publication , it is an abstract submitted to: Congresso Brasileiro de Hematologia, Hemoterapia e Terapia Celular -...
17 July 2024 4,035 15 View
In my research on developing an integrated training management system for industrial port complexes, I'm investigating the role of automated notification systems. This is crucial because these...
09 April 2024 1,349 0 View
I need to do some analysis on my personal computer and unlike the Quantstudio software, I can't find a free version of the 7500 Real-Time PCR software
11 May 2023 4,295 1 View
I'm trying to make a co-culture with AGS cells and B cells, and we want to do a sorting. Does anyone here know a good surface marker? this cell line doesn't express catenin beta 1, e-cadherin and...
09 April 2021 4,308 4 View
I made an cytokines expression curve, in 7dpi and 14 dpi Interferon gamma have a expression peaks, but at 28 dpi I observed an expression below the mock / control. Could this result be only...
01 April 2021 8,924 3 View
I need to purify monocytes isolated from human peripheral blood using the plate adhesion methodology. However, I only have DMEM in the laboratory. I did a pilot and the cells did not adhere...
09 December 2020 2,750 3 View
All available protocols use the Percoll gradient to isolate T cells, unfortunately we only have Ficoll 1,077 available at the moment. Does anyone experience you with this type of isolation?
13 October 2020 7,804 3 View
I have read many books and articles which consider the XIIth century a moment of great changes in the culture of Middle Ages, a moment where the writings and manuscripts become more used and...
05 December 2016 3,252 33 View
In order to perform a fMRI ROI analysis we need some prefrontal cortex region masks. Particularly, we are interested in the vmPFC, dmPFC, dLPFC and vlPFC. It seems that are a lot of different...
14 July 2016 1,530 7 View
I am doing research in education / psychology, and I am using Interest as a motivational variable. I am trying to assess how situational interest might trigger a more developed interest in a topic...
14 July 2015 7,496 4 View
In order to show people the beauty of control and enhance enthusiasm for learning control theories, are there any good simple systems or platforms to recommend?
05 August 2024 10,034 1 View
Hi guys If anyone is currently working on aging cells, you guys would like to give me some advice. I'm testing against biomarker (SA-beta-Gal), I encountered a false positive in the control group...
02 August 2024 6,735 1 View
I am currently researching the impact of environmental toxins on children's health and would greatly appreciate insights from experts in the field. If you are an expert or researcher working on...
02 August 2024 4,474 2 View
I have seen plenty of existing works on applied Reinforcement Learning (RL) policies for optimized scheduling in IoT networks including Q-learning, DQNs, and the newer ones including PPO for...
01 August 2024 8,754 2 View
We have a lateral flow test on Sartorius 140 NC. The conjugate is gold-monoclonal antibody. Using different control lines (GAM, protein A, protein G) we get a very strong leading edge with reduced...
31 July 2024 4,510 3 View
Hello, I have a little problem. Well, right now I only have the DNA aptamer sequence. I went to see the 2D model form with DNA folding. After that, I used RNA Composer to simulate in 3D and used...
30 July 2024 6,794 0 View
Their is new research published in the biological control journal, and I am one of the authors. I want to add it to my account at researchgare. How can I do this?
21 July 2024 7,545 0 View
I'm trying to use cassowary footprint to estimate their population since their footprints are distinguishable from other animal on the field. is it really possible to do that? since it's really...
18 July 2024 3,729 7 View
I do not know much about FTIR! The material Benzethonium Chloride USP was compared to a reference standard in regards to FTIR, as per the USP testing: Identification B. Infrared absorption (FTIR),...
16 July 2024 6,918 7 View
You have been appointed as the supply chain manager for a medium-sized hospital facing challenges in its supply chain operations. The hospital aims to improve material management, optimize...
15 July 2024 4,120 4 View