Ct of target gene in Untreated condition is undetermined, how does performing Livak's method (DDCt method to generate fold change values (2–DDCT)?
I need to purify monocytes isolated from human peripheral blood using the plate adhesion methodology. However, I only have DMEM in the laboratory. I did a pilot and the cells did not adhere...
09 December 2020 2,555 3 View
What are the most importants compounds from Jacaranda caroba and Calendula officinalis?
03 February 2015 9,914 7 View
Dear Dr. Cai, my name is Simone Prospero and I work in the team of Phytopathology at the Swiss Federal Institute for Forest Snow and Landscape Research (WSL;...
01 March 2021 1,133 1 View
The following code (see 1st 2 images attached) is used to produce PID controller values that are designed to control the system (G). The code finds the PID controller values (noted as k) by using...
28 February 2021 6,560 14 View
If I want to compare characteristics of 2 groups of patients, one group is recruited from a random sample of the general public while another group is from a sample with a fixed gender (male to...
26 February 2021 3,526 3 View
Good day to all, I have no experience with light emitting diodes. I am building a glass mini flume to demonstrate the photo degradation of Methylene Blue in water running over self cleaning...
26 February 2021 4,026 5 View
Has anyone ever tried to deliver CO2 with a CGA 300 regulator? I know that a CGA 320 regulator is standard for CO2. The issue is that I currently do not have any free CGA 320 regulators. I also...
25 February 2021 3,292 3 View
Hello ! In a work, I have to benchmark different algorithms to fill in missing values in time series. I insist on the fact that this is imputation and not forecasting. In my case, I have access...
24 February 2021 7,470 7 View
Hi, I am having trouble with a problem, in the field of Optimal Control and the generation of optimal time-series. Let's consider a system, whose dynamics are represented by dx/dt =...
24 February 2021 9,105 2 View
Hi everyone, I'm researching about mobile learning in higher education. Due to the small number of the students in the semesters, I cannot have a experimental and control group together. I'm...
22 February 2021 3,653 11 View
My real system (buck converter) can only take an input of 0 to 1 (duty ratio) and I need to constrain the system so the controller action keeps within this bounds. How can I do this? The code for...
22 February 2021 2,125 3 View
I ran RT-qPCR for a control and a treated group and it appears that my gene of interest was not expressed at all in any of the subjects in the control group. So I was wondering if I could...
20 February 2021 6,243 10 View