Fabrícia Lima Fontes

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Questions related from Fabrícia Lima Fontes

Is there a free downloadable version of the Applied Biosystems 7500 Real-Time PCR Software?

I need to do some analysis on my personal computer and unlike the Quantstudio software, I can't find a free version of the 7500 Real-Time PCR software

12 May 2023 4,267 1 View

Can the expression of a cytokine fall below the mock/control after the resolution of a viral infection?

I made an cytokines expression curve, in 7dpi and 14 dpi Interferon gamma have a expression peaks, but at 28 dpi I observed an expression below the mock / control. Could this result be only...

02 April 2021 8,901 3 View

Is it possible to grow human monocytes in DMEM?

I need to purify monocytes isolated from human peripheral blood using the plate adhesion methodology. However, I only have DMEM in the laboratory. I did a pilot and the cells did not adhere...

10 December 2020 2,704 3 View

Isolation of T cells from adult mouse brain is possible with ficoll gradient?

All available protocols use the Percoll gradient to isolate T cells, unfortunately we only have Ficoll 1,077 available at the moment. Does anyone experience you with this type of isolation?

14 October 2020 7,781 3 View

QPCR expression difference

Ct of target gene in Untreated condition is undetermined, how does performing Livak's method (DDCt method to generate fold change values (2–DDCT)?

01 January 1970 6,794 0 View