I have isolated the RNA from the sciatic nerve and DRG approximately 3 weeks before and stored at -80 with 1xTE buffer. Then before attempting to do the RT PCR for the cDNA synthesis, interestingly I have noticed that nano drop measurement is showing different but higher concentration like 60% increased in case of every sample. I am wondering it had happened in case of others and what are the consequences for example using the cDNA in the qPCR?

Thank you.