Ulfuara Shefa

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Questions related from Ulfuara Shefa

Is there a way to correct the A260/230 ratio if it falls below the acceptable range following RNA extraction from tissue?

In my RNA extraction from mouse sciatic nerve and DRG tissues, I found that the A260/A230 ratio was lower than the recommended range of 2-2.2 after measuring RNA concentration with a NanoDrop. Is...

07 September 2024 9,859 1 View

QPCR data Analysis-Delta-Delta Ct Method, Control Fold Change can be more than 1?

After analyzing the qPCR data, I found that the control fold change averages are 1.8, 2.5, and 1.2. Typically, discussions suggest that the control fold change average should be 1, but in my case,...

03 July 2024 7,914 0 View

Can the concentration of RNA increase upon storage at -80 degree at 3 weeks?

I have isolated the RNA from the sciatic nerve and DRG approximately 3 weeks before and stored at -80 with 1xTE buffer. Then before attempting to do the RT PCR for the cDNA synthesis,...

04 April 2024 4,388 1 View