PVDF vs PES, which membrane is better at retaining extracellular vesicles during filter sterilization of biofluids before and after the isolation process?
In the primer validation, with the serial dilution of the cDNA, I have a melt curve with a single peak. However, when I do the gene expression using control and treatment samples (I am evaluating...
17 August 2020 2,793 5 View
Hi guys ! I have a fundamental question regarding the fitness criterion when performing a model estimation for system identification. This far, I have obtained a good model for a driving...
28 October 2019 3,656 1 View
Hi guys, Reading my question title surely has given at least some of you a flashback on their experiences during the estimation of a nonlinear system model. I hope to get some tips, tricks and...
18 September 2019 6,417 3 View
how does the pressure effect crystallinity of polymers? if samples dried in vacuum will have higher crystalline structure or lower or stays the same ? i'm guessing that lower pressure will have...
31 March 2019 3,196 4 View
What other models i can use other than: wembel-di domineco model, tauc plot, urbach tail and energy gap from absorption/transmission For thin films using spectrometer T&R&A I tried using...
16 October 2018 5,473 1 View
I have Independent variable which the diversity of board of director and the dependent variable is the strategic change and after that I want see how the strategic change can affect the firm...
02 September 2016 1,170 9 View
Non-Diversified firms
01 January 1970 9,699 1 View
Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...
01 March 2021 8,169 2 View
Hello! I would like to know if there is a way to flush/remove all the cells out of a tissue and keep only the extracellular matrix, in order to analyse its composition using e.g. proteomics....
14 February 2021 1,602 4 View
Hey guys, I am currently doing tube formation assay on matrigel coated coverslips, and i wonder if there is any possibility to get rid of the meniscus formation? I have only a small part of the...
09 February 2021 6,979 3 View
Can anyone provide or recommend a detailed protocol for preparing extracellular vesicles for SEM? Most protocols that I have found that list the necessary reagents and individual steps are for...
02 February 2021 4,475 3 View
Hi all, I have to do a phenotyping study on cell culture derived EVs, in particular I should stain them using primary labelled ABs anti-EVs markers before proceeding to analyze them through...
25 November 2020 4,601 1 View
Hi all I use psychopy 3 to show mice a visual stimulus on an LCD screen while preforming acute extracellular recordings in vivo. I understand that one should use a spherical wrap to the stimulus...
22 November 2020 7,905 2 View
Hello, I am trying to set up an in vivo recording system in mice brain. Which company do you recommend? Neuralynx? TDT? NeuroNexus? Which one do you guys use and why? Please let me know. Thank you!
12 November 2020 2,508 1 View
Recently, there was a problem with my qpcr results. I want to try more methods of extracting EV from the cell medium. At present, I am using Invitrogen's kit(Cat4478359) to extract exosomes. Can...
28 October 2020 2,961 3 View
I read many papers about exosome isolation from fresh blood or frozen plasma, but not for frozen whole blood. I am wondering whether can I get exosomes from cryopreserved whole blood? One of my...
26 October 2020 5,166 3 View
Hi, I am trying to create a histology based finite element model of human soft tissue. In order to model the viscoelasticity at the cellular level, I need to know the dynamic and bulk viscosity of...
19 October 2020 8,729 1 View