PVDF vs PES, which membrane is better at retaining extracellular vesicles during filter sterilization of biofluids before and after the isolation process?
I am trying to transform pichia pastoris GS115, but when I run the negative control (the cells without the DNA) it is also growing in the selection medium Minimal Dextrose Medium:1.34% YNB 4 ×...
04 July 2023 920 6 View
I am evaluating the isolated and combined effects of two stressors on physiological and biochemical parameters of fish. I analyzed different markers (eg antioxidant enzymes, DNA damage,...
17 April 2023 7,549 2 View
I need a dataset related to Vehicular ad hoc Networks for Reflection-based DDoS attacks. Any idea or suggestion is welcomed. Thanks you!
24 June 2022 6,492 3 View
Hi, we are having a problem with protein transfer. We used the PVDF membrane (0.45 um) activated by methanol (20s). The pre-stained marker and a recombinant protein that we used as a control were...
23 March 2022 8,644 3 View
Hello, i'm a novice researcher and i m having a problem analyzing an article i do understand the methodology used and results but i can't figure out the epistemological position or the reasoning...
22 July 2021 744 2 View
I want to download 3d structures of two proteins of Acinetobacter Baumannii but am finding difficulty on PDB. can anyone guide me how to view or download 3d structures of my proteins (1....
02 December 2020 4,678 4 View
In the primer validation, with the serial dilution of the cDNA, I have a melt curve with a single peak. However, when I do the gene expression using control and treatment samples (I am evaluating...
17 August 2020 3,028 5 View
If I find this type of printer, can it print very precision pieces?
17 February 2020 2,854 2 View
Hi guys ! I have a fundamental question regarding the fitness criterion when performing a model estimation for system identification. This far, I have obtained a good model for a driving...
28 October 2019 4,026 1 View
Hi guys, Reading my question title surely has given at least some of you a flashback on their experiences during the estimation of a nonlinear system model. I hope to get some tips, tricks and...
18 September 2019 594 2 View
Is it appropriate to place the ATG codon in front of the gene of interest, since there is a secretion signal that has its own ATG in front of this gene? I need my protein of interest to be...
10 July 2024 3,050 5 View
Hello to everyone, I'm trying to extrtact total RNA from sEVs. I made a first experiment starting from 2x10^10 sEVs quantified by NTA but the RNA obtained was very low (under 20ng/ml) Do you have...
18 June 2024 2,799 4 View
Hi, I am doing western blotting of isolated extracellular vesicles, and I have albumin and calnexin contamination that I don't know why. Is there any way to avoid it?
16 June 2024 9,907 4 View
Please give some insight on this
06 June 2024 2,016 2 View
Ultracentrifugation is the most widely used method for isolating extracellular vesicles (EVs), despite the significant structural damage that EVs suffer during the process.
16 May 2024 9,432 1 View
In the younger boy there is no ejaculate because the prostate and seminal vesicles are not yet functionally developed, and in the female those glands are rudimentary and never develop. (Alfred Kinsey)
29 April 2024 6,483 0 View
I tried to inhibit extracellular microvesicles(MVs) secreted from cells, through using Rock inhibitor(Y27632), calpeptin and several siRNAs(siCDC42, siTSG101, siARF1), but failed to decrease MVs....
23 April 2024 7,369 0 View
Dear Colleagues, We plan to isolate and characterise extracellular vesicles released by dying cells during radiotherapy of head and neck squamous cell carcinoma. Given the duration of...
14 April 2024 494 3 View
I am planning to study extracellular vesicles, and to do this I need cells with a fluorescent membrane (GFP, Tomato, etc.), do you guys have any suggestion of lentivirus vector? Thanks!
02 April 2024 1,944 0 View
Hello dearest EV people! I want to plate my isolated EVs on coated glass coverslips and image them with confocal microscopy for CDs and other EV markers. I wonder if anyone tried a normal IF...
21 March 2024 3,344 1 View