Hi,

I have cleaved the GST fused His-Tag from my expressed protein at the N terminal using Enterokinase enzyme. My protein of interest (17 to 20 kDa) is also having 6X His at its C terminal, while  the enzyme Porcine Enterokinase also have 6×His tag at the N-terminal.

1. GST-fused His Tag, 2. Enterokinase enzyme having 6X His tag at the N- Terminal, 3. Protein of interest (17 to 20 kDa) having 6X His tag at the C- Terminal

Now how to take out, only my protein of interest from this pool

I need only the third one for my western blotting without the other two.

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