Krishna Raja Muthuraman

3 Questions 10 Answers 0 Followers

Questions related from Krishna Raja Muthuraman

Does the His tag at C terminal of recombinant protein of ~20 kDa size affects in western blotting/ELISA?

Hi, My recombinant protein of size ~ 20kDa has a 6X His tag at its C-Terminal end. This recombinant proteins` purpose is to serve as a diagnostic antigen looking for antibody in the infected...

07 July 2017 1,831 8 View

Purification / separation of recombinant protein of interest from the digestion pool

Hi, I have cleaved the GST fused His-Tag from my expressed protein at the N terminal using Enterokinase enzyme. My protein of interest (17 to 20 kDa) is also having 6X His at its C terminal,...

11 November 2016 9,401 4 View

How to Immunoscreen a cyst cDNA library?

I have constructed a cDNA library of Taenia solium cyst using clontech (smart cDNA library construction kit). I have to immuno screen the library. Kindly suggest further methodologies regarding...

07 July 2016 3,787 0 View