Prunus species determination?

07 January 2019 0 8K Report

Hello to everyone. I want to determine the types of prunus samples I do not know.

Should I choose ITS 1 and 2 as pcr primers? can I determine types if I make a bi-directional sequence with these primers ?

Thanks for your reply.

Badges
Science topic

More Utku Kutoglu's questions See All

Would Hyaluronic Acid be good choice as a ligand for using in Mesoporous Silica Nanoparticles for breast cancer treatment by PhotoDynamic Therapy?

We are trying to design Mesoporous Silica Nanoparticles for breast cancer treatment by PhotoDynamic Therapy. And we are challenging with a question that Which ligand should we add onto the...

24 April 2022 1,443 1 View

Can we run a reliability analysis with non-normally distributed data? Is there an alternative to reliability analysis for non-normal distribution?

I want to learn the reliability coefficient of a scale I used in my study (an assignment for my experimental psychology class). I read about how to find Cronbach alpha, I can run a reliability...

18 December 2020 7,549 4 View

How to Remove Silanization Agent (TFOCS) from Silicon Wafer Mold?

Hi all, Like many of you, I was coating my Si wafer molds with a silanization agent (TFOCS) using vapor deposition technique to facilitate easy removal of PDMS. However, the last time, I...

02 May 2019 4,477 2 View

Endothelial Cell Alignment Perpendicular to the Flow?

Hello everyone, Like all of you know, endothelial cells align parallel to the direction of the flow as a response to flow shear stress. There are plenty of papers about this in the literature....

02 July 2018 6,042 17 View

How to prepare methyl cellulose solution in mammalian cell culture media?

Hi all, I am planning to use 1-2% methyl cellulose (15 cP, Sigma) to increase the viscosity of my cell culture media (EGM, Sigma). In the website of the sigma, they describe 2 methods to prepare...

30 March 2018 9,695 3 View

Difference Between FEA Result and Hand Calculation in Beam Bending?

Hi all, I was trying to model deflection in a rectangular cross-section beam fixed at both ends. There is a Pressure Load acting on the beam (uniformly distributed along the beam). In such...

25 November 2017 3,418 18 View

Where can I find polyurethane foam with high PPI values?

I am looking for polyurethane foam/sponge material with pore sizes up to 1000 ppi. In other words the pore size should be in the range of 20 micrometer to 100 micrometer. Thank you in advance.

08 June 2017 1,607 2 View

What is the bottom limit for Stoke's Drag assumption in terms of particle size?

Hello everyone, I was trying to model concentration gradients in a flow-free diffusion chamber using COMSOL. I wanted see what would be the effect of gravity on the concentration gradients that I...

06 June 2017 2,925 3 View

What are alternative repositories like arXiv.org?

I am looking for alternative repository sources like arXiv.org where I can upload my open access papers. Do you have suggestions on that? Thanks!

03 April 2017 6,648 19 View

Has anyone made a baseline correction to h-nmr data of petroleum fractions?

Which ppm points you use for to correction?

08 August 2016 7,907 3 View

Effect of chemical properties of homemade almond milk for storage time?

explain the chemical properties and dielectric properties of homemade almond milk.

26 May 2024 5,938 0 View

What is the scientific basis for many amino acid-based product to say "Do not apply foliarly to plum trees as there is risk of phytotoxicity"?

I have found many products in the market stating this, but I have not found any published article that describes this phytotoxicity issue with Plum trees that have been sprayed with an amino...

26 March 2024 7,522 2 View

Help adapting the root squash method to observe mitotic events in shoot apical meristems?

Hello, I am trying to use the root squash method to observe mitosis in almond (Prunus) shoot apical meristems, but I'm not getting clear staining of chromosomes. This is the protocol I'm using so...

26 April 2022 2,869 4 View

Why some of my DNA samples are not working with ISSRs?

Almost my 70 samples worked just fine with ISSR (AG)8. However, the same condition did not work with other samples. My isolates are about trunk fungal diseases of almonds such as Neoscytalidium...

24 February 2022 3,140 4 View

Is this a leaf miner on Apricot?

I suspect it to be Apricot leaf miner Phyllonorycter sp. Like the apple blotch leaf miner that is known to attack several prunes. Can anyone help me in the identification of the same? I am...

02 August 2021 8,215 5 View

How can I get Prunus dasycarpa seeds?

For experimental work I would like to grow Prunus dasycarpa. Where can I find seeds?

13 January 2021 6,529 3 View

What are the best steps to rehabilitate a soil from any kind for the use in the agricultural field?

I Hope you receive my email and you are in the best of Health and Joy. I just want to see if anyone can work on a study project, that will help a great number of people. In summary: one of...

21 September 2020 1,408 4 View

What is negative rhizosphere effect and its implications on long term crop performance?

Perennial crops survive for 40-50 years in tandem , without experiencing any decline in productivity . But , many a times , this is not the case , for eaxmple , apple , pear, peach , tea and...

10 September 2020 5,330 3 View

How to get information concerning chilling requirement for temperate fruit trees to be cultivated in semi-coastal regions of West Bank - Palestine?

Dear All, I need to calculate the needed chilling hours or portions for peaches and nectarines we plan to cultivate in Tulkarem region in West Bank - Palestine. The region is about 120 - 150 m...

24 July 2020 7,710 0 View

Do you have information about the available free program for determining the area of leaves using a photo or image scanner?

It is necessary to quickly and in large repetition determine the area of the leaves of Prunus tenella in different lighting conditions.

21 June 2020 2,457 5 View