How can I get Prunus dasycarpa seeds?

More Ákos Mendel's questions See All

Why is Fc tag only present on a fraction of my produced recombinant proteins?

Hi! I have trouble finding literature on this. Can someone please share some information on what could be the cause of a glycoprotein-Fc-tag fusion protein being expressed in HEK cells missing its...

19 October 2021 3,080 3 View

How much poly-A RNA should I use for transcriptome sequencing using minION (ONT)?

I extracted ~87 ug of total RNA from mushroom samples using TRIzol (Invitrogen) and I got ~1,2 ug of poly-A RNA using the Oligotex kit (QIAGEN) for mRNA purification. The protocol of direct cDNA...

31 March 2021 2,063 0 View

Any hint on adding a phylogenetic tree tip labels in MEGA X or FigTree 1.4.4 ?

Hello, how do I make a neat phylogenetic tree, such as the one you can see in the attachment (Fig1.jpg) ? Notice how there are multiple taxa displayed under each other on a single terminal branch...

25 March 2021 9,772 3 View

Why degrade my cDNA during the second strand synthesis reaction?

Dear All, I would like to ask some help in second strand synthesis. I’m currently working on a project which require the conversion of a single stranded RNA virus genome (i.e. a norovirus) into a...

16 March 2021 1,999 2 View

Is it strictly recommended to run samples in duplicate during SNP genotyping analysis?

We are using AppliedBiosystems TaqMan® SNP Genotyping Assays. Is it ok not to run the samples in duplicate to increase sample size?

02 February 2021 9,110 3 View

Apricot database - where to search?

I work in apricot research and we have a breeding program for abiotic stress tolerance. I collect data from the cultivars i could use in this program individually, but there are several hundred...

01 February 2021 584 3 View

Can You predict the estimated fruit size of a cultivar from average leaf area?

In the early non-bearing years, if we measure the average leaf area (not the LAI!), can we screen the the seedling population for fruit size?

13 January 2021 1,550 4 View

Can MaxEnt use background points with one or more missing environmental data?

I know that the allowpartialdata parameter, which is by default false, can be set to true, and in this case presence points with one or more (but not all) missing environmental data are not...

26 June 2020 464 0 View

Is there something I can do to increase the chances of successful ISSR bands amplification during PCR ?

Hello, I have a following issue: I extracted approx. 20 plant DNA samples from dry leaves (old herbarium items) using Qiagen DNeasy Plant Mini kit. When I run a PCR for housekeeping gene (alpha...

22 March 2020 2,842 6 View

Abcam anti-RBP4 and anti-Thy1 antibodies for brain tissues - any experiences?

Hey everyone! Have anyone used the rabbit anti-Rbp4 (ab109193) and rat anti-Thy1 (ab85352) antibodies for fluorescent immunoshitochemistry in mouse brain slices? Does anyone have any experience...

14 May 2019 1,429 0 View

What is the most effective way to sterilize camelina seeds?

I'm working on camelina seed sterilization and have some doubts about the process. I followed a protocol used by my lab mate, but I'm unsure about a couple of things: Is it safe to use 10% bleach...

02 August 2024 1,178 3 View

When should I stop watering A.thaliana for collect their seeds?

I have been following Rivero et al 2014 protocols, but I am not sure when I should stop watering because my plants are completely yellow but the siliques are not 80% brown. Any recommendations?...

30 July 2024 2,301 7 View

How should I prevent mould contamination on small seeds in a petri dish exposed to warm temperatures?

Hello all, Recently as part of a research project at university, we have been studying the effects of light and temperature on eucalyptus seed germination. In our initial run of trials we...

24 July 2024 8,850 4 View

HEC 1A & HEC1B Cell Lines?

Hi, Kindly guide me that how many cells of HEC1A & HEC1B Cell lines should I seed for Wound healing assay and which plate type is recommended 6, 12 & 24?. Articles suggested mainly 24...

20 July 2024 4,143 2 View

How to improve my plaque assay for influenza virus?

I used MDCK cells for my plaque assay for influenza virus and seeded 950,000cells/well in 6-well two days before infection. The virus was diluted in FBS-free MEM with 0.5ug/ml TPCK-trypsin. Here...

11 July 2024 5,669 5 View

How to convert protein percentage into kg/ha?

I have protein percentage and Seed yield per hectare data, how to calculate protein content kg per hectare.

11 July 2024 9,045 4 View

How can I determine the percentage of cell viability of cells seeded in 96 well plate ?

I am currently testing the effect of bacterial filtrates on cancer cells , after seeding the cells I tested the bacterial filtrates against them , and got images of all 96 wells using inverted...

10 July 2024 7,145 2 View

I seed my cell in Opti-MEM?

I seed my cell (KG1a )in opti-mem media for transfection (without lipofectamine as control ) and after 2 hours I add FBS 10 ul in each wells all my cells after 24h in control wells or with...

07 July 2024 407 0 View

Do the SHSY5Y cells tend to form multiple layers over each other when highly confluent?

I have attached pics of the SH-SY5Y cells within a seeded plate after reaching >80-90% confluence. Are these dark clumps of multiple growing cell layers? Or dead cells? Any clue? many thanks.

01 July 2024 8,361 3 View

How to transfer a full grown cell monolayer to another plate?

Hi everybody, i need to pick your brains: i work with an adhesive fish cell line which forms a konfluent monolayer. I want to test an adsorbant substance, therefore i want to saturate the cell...

30 June 2024 9,300 0 View

Andrew Paul McKenzie Pegman

You can request a sample from Kew Garden seed bank :)

Ákos Mendel

Thank You!

M Wanlambok Sanglyne

You can request for samples either from your local government or privately owned seed banks. Universities with Botanical Gardens sometimes do preserved seeds, this can be an option too.