Can a His tagged protein be eluted and dialysed in a buffer other than that of standard Ni-NTA elution buffer?

A recombinant his tagged protein (Size: 27 KDa Theoretical pI: 7.85) is eluted fine with 50 mM NaH2PO4 pH 8.0, 500 mM NaCl and 250 mM Imidazole. Precipitation occurs during dialysis with same...

02 March 2019 4,210 4 View

Doesnt the reaction volume matter for sequential digestion with two incompatible enzymes?

11 December 2016 7,345 5 View

Can ELISA be used as a simple test for the presence or absence of antigen?

07 August 2015 7,397 7 View

Does anyone have experience with PCR product as template for in vitro transcription?

I am searching for options of templates for PCR reaction, to use the resultant PCR product as a template for in vitro transcription. I came through options such as plasmid containing gene of...

06 July 2013 4,200 4 View

Is there any special technique or microscope to take pictures of stained embryos?

I am doing WMISH experiments and usually get the stained embryos. I am not able to take the picture of my results. When I observe my embryos under microscope it looks like in the picture attached....

08 September 2012 9,777 7 View

Does anyone have experience of using Agdia Antibody for western blotting?

01 January 1970 3,921 1 View

Probiotics Regulations in India

Are there any recent additions or modifications to the "ICMR-DBT GUIDELINES FOR EVALUATION OF PROBIOTICS IN FOOD" (2011). Are there any other regulatory guidelines governing the commercialisation...

01 January 1970 1,729 3 View

Interested to stain mice tissue with WGA 488 conjugate, anybody has a protocol to recommend?

Interested to stain mice brain tissue with WGA 488 conjugate, appreciate if anyone willing to offer me some guidance or suggestions! Thank you!

28 February 2021 3,951 2 View

Anyone know of a good progesterone antibody?

I am looking for a good progesterone antibody to quantify levels of progesterone in zebrafish. Applications would be ELISA, and immunohistochemistry.

28 February 2021 5,929 2 View

B-mercaptoethanol (BME) protocol?

Hi everybody, I have been running a multiplex IHC which allow me to stain the same tissue with different markers (Primary Ab + Secondary conjugated Ab) (AF487, AF555, AF647). Since I would like to...

27 February 2021 5,028 2 View

Why do my MCFs not form monolayer?

Hello, I am looking for some expertise on culturing MCF7s. I purchased them from ATCC and I have been following all media and culture conditions. Media is EMEM with 10% FBS and 10ug/mL of...

27 February 2021 3,904 2 View

Is there anyone who experienced with cytotoxic effect of Hoescht 33342 dye?

Is Hoechst toxic to cells? In general, it is known as non-toxic. I am working with dsRED MDA-MB-231 cell line. I applied it for my PDMS-based microfluidic platform, for this reason, I incubated...

26 February 2021 9,119 3 View

Why my agarose gel staining sometimes looks not homogenic but like gradient (see the figure)?

I am worried about this overexposure of the upper part of the gel in the picture. Is it possible that this is the result of too much concentration of ethidium bromide? Why is there such a big...

25 February 2021 8,140 3 View

Markers of mitochondrial activity in FFPE tissues?

Hello everybody, Does someone have any experience with assessing/extimating mitochondrial activity on FFPE tissues? Precisely, no TOM20, ATP5B, etc: we are trying to get an approximative idea of...

24 February 2021 4,618 4 View

Can a Golgi-stained dendritic spines picture processed by Image J be directly used for publication?

24 February 2021 3,348 2 View

Is there an appropriate CD8 antibody for IHC-P for staining human breast cancer tissues?

Hello, We are planning on staining Formalin-fixed paraffin breast cancer tissue sections (FFPE) with CD8 Ab via immunofluorescence (Tissue IF). Based on our existing protocol and imaging...

24 February 2021 9,578 4 View

Reagents for histology slides?

Dear all, I am planning to study histology and want to do that at home. I have only stained a blood smear with Giemsa but now want to try a wider range of animal tissues. What reagents will I...

24 February 2021 5,430 3 View