Nagganatha Suganthan Ravi

8 Questions 4 Answers 0 Followers

Questions related from Nagganatha Suganthan Ravi

Can a His tagged protein be eluted and dialysed in a buffer other than that of standard Ni-NTA elution buffer?

A recombinant his tagged protein (Size: 27 KDa Theoretical pI: 7.85) is eluted fine with 50 mM NaH2PO4 pH 8.0, 500 mM NaCl and 250 mM Imidazole. Precipitation occurs during dialysis with same...

03 March 2019 4,286 4 View

Doesnt the reaction volume matter for sequential digestion with two incompatible enzymes?

I am sequentially digesting a plasmid with two incompatible restriction enzymes (NEB). The NEB protocol recommends to always carry out the first digestion with the enzyme that requires the lowest...

12 December 2016 7,385 5 View

Can ELISA be used as a simple test for the presence or absence of antigen?

I understand that ELISA is used to titre the amount of antigen present in a test sample based on the intensity of the chromogenic reaction of peroxidase on peroxide. So it means that the...

08 August 2015 7,434 7 View

Does anyone have experience with PCR product as template for in vitro transcription?

I am searching for options of templates for PCR reaction, to use the resultant PCR product as a template for in vitro transcription. I came through options such as plasmid containing gene of...

07 July 2013 4,242 4 View

Protocol request for mounting stained embryos in BB:BA mixture

Can anyone recommend a protocol for mounting the stained embryos in BB:BA mixture starting from the 4%? PFA fixation after the staining

09 September 2012 8,542 1 View

Is there any special technique or microscope to take pictures of stained embryos?

I am doing WMISH experiments and usually get the stained embryos. I am not able to take the picture of my results. When I observe my embryos under microscope it looks like in the picture attached....

09 September 2012 9,813 7 View

Does anyone have experience of using Agdia Antibody for western blotting?

I need to perform western blotting of a purified plant viral protein using specific antibodies against the virus. I searched for purchasing the virus specific antibodies and found them in Agdia. I...

01 January 1970 3,962 1 View

Probiotics Regulations in India

Are there any recent additions or modifications to the "ICMR-DBT GUIDELINES FOR EVALUATION OF PROBIOTICS IN FOOD" (2011). Are there any other regulatory guidelines governing the commercialisation...

01 January 1970 1,768 3 View