We have had ample experience (and we had successes) with the alkaline comet assay, however the neutral assay seems more challenging.

I am no longer running it, however I am trying to help a colleague from our Institution, who has encountered 'fried eggs' as opposed to comets with various degrees of damage/tails.

He has to gently trypsinize his cells in culture.

Can anyone share a wonderful protocol? thanks in advance.