Hi,
I am looking for a detailed protocol where I can measure both mitochondrial and cytosolic calcium dynamics using live cell imaging? The buffer conditions and the optimal ionophore concentration to be used?
thanks
devi
Hi, I wanted to do bisulfite treatment on tRNA (length 72bp) and design taqman probes for methylation specific PCR. Have anyone done this before and could you please let me know the things which i...
01 February 2016 3,156 0 View
I need a very good protocol for competition binding assay. I have a complex and a third protein. I expect the third protein to dissociate the complex by binding with one partner of the complex....
10 November 2014 6,069 3 View
I am trying to purify two proteins. Protein 1, is around 16kd, sequence verified, expression verified via western blotting. problem is induction. it is not induced. i tried various concentrations...
09 October 2014 2,620 10 View
I am trying to subclone gene of my interest along with tag from pcdna3.1 to pET28b vector. As the insert has its own ATG, gene of interest and fusion tag in frame, I want to cut the whole lot and...
08 September 2014 634 6 View
See above.
05 June 2014 3,659 8 View
Does anyone tried to do nucleofection with AMAXA by Lonza with lower than recommended amount of buffer in the cuvettes (100 ul)?
07 August 2024 4,616 0 View
Currently, when I run SDS-PAGE, I don't see any bands at all, even though I used the same material just a day ago and it worked fine.... In our lab, we dilute the 10X running buffer to 1X and...
06 August 2024 5,373 2 View
Hello everyone, I'm encountering an issue with my electrochemical impedance spectroscopy (EIS) measurements and would appreciate some insights. Experimental Setup: Electrodes: Gold interdigitated...
05 August 2024 3,783 2 View
Is it the "elution buffer" or the "dialysis buffer"? Note: I'll be using NanoDrop OneC
01 August 2024 967 3 View
Hello everyone! How can i prepare EDTA buffer solution (pH 8.2) to determine GSH levels? Thank you in advance.
29 July 2024 4,374 1 View
I want to preserve the sample leaves for the LM study of the stomata and conidia analysis. Whether 2.5% glutaraldehyde in 0.05 M phosphate buffer with pH 7 can be applied for preservation.
29 July 2024 8,560 0 View
// interested in the difference between floating events and short circuits.
22 July 2024 6,565 0 View
Our lab has extracted bacterial DNA samples using Qiagen DNeasy kit and eluted the samples in AE buffer. However, this buffer contains 0.5mM EDTA not suitable for downstream application. Any ideas...
13 July 2024 1,675 3 View
Commonly, we prepare 1.0% of agarose gel with 0.4g agarose powder and 40ml 1X TAE buffer. if I would like to prepare a 1.2%% of agarose gel, is it I need to add 0.48g agarose powder and 120ml 1X...
12 July 2024 2,058 5 View
I performed CV of Cu-MOF in a buffer solution of pH 8, and we did not find any peak corresponding to Cu2+ to Cu+. What is the reason behind this? Please give your valuable suggestions.
08 July 2024 2,388 4 View