Hi,
I wanted to do bisulfite treatment on tRNA (length 72bp) and design taqman probes for methylation specific PCR. Have anyone done this before and could you please let me know the things which i have to take care of while designing the probes?
Hi, I am looking for a detailed protocol where I can measure both mitochondrial and cytosolic calcium dynamics using live cell imaging? The buffer conditions and the optimal ionophore...
11 December 2017 5,633 0 View
I need a very good protocol for competition binding assay. I have a complex and a third protein. I expect the third protein to dissociate the complex by binding with one partner of the complex....
10 November 2014 6,069 3 View
I am trying to purify two proteins. Protein 1, is around 16kd, sequence verified, expression verified via western blotting. problem is induction. it is not induced. i tried various concentrations...
09 October 2014 2,620 10 View
I am trying to subclone gene of my interest along with tag from pcdna3.1 to pET28b vector. As the insert has its own ATG, gene of interest and fusion tag in frame, I want to cut the whole lot and...
08 September 2014 634 6 View
See above.
05 June 2014 3,659 8 View
I'm cloning a fragment of 3200 nts into plasmid. The cloning was successful, however, 02 amino acids were mutated. Now I want to fix these 02 aa by site-directed mutagenesis technique using...
08 August 2024 4,645 2 View
After performing symmetric PCR, PCR purification was performed. Afterwards, asymmetric PCR was performed using the PCR purification product as a template, but no ssDNA band was confirmed in the...
08 August 2024 1,668 3 View
There are a huge number of methods for studying objects in space, according to the senses (and not only). Mechanical, thermal, optical, acoustic, electrical, magnetic, based on particle beams,...
06 August 2024 7,102 0 View
i have sorted anti-NP specific plasma cells from bone marrow of C57BL/6 mice at certain times after immunization with variable counts and isolated total RNA using TRIZOL method for RT-PCR using...
05 August 2024 8,835 1 View
As described in the Multifractal detrended fluctuation analysis (MFDFA) algorithm, it at first calculates the profile of the time series, and then other steps are operated on the profile....
05 August 2024 9,366 2 View
I need the python code to forecast what crop production will be in the next decade considering climate and crop production variables as seen in the attached.csv file.
05 August 2024 2,977 3 View
I want to know how do you make real time pcr solation ? is there any machine to make it? thanks for answering
05 August 2024 1,660 0 View
Hello everyone, I am currently working on a research project that aims to integrate machine learning techniques into an open source SIEM tool to automate the creation of security use cases from...
04 August 2024 3,196 2 View
I have been attempting to extract DNA from Bacterial, Fungal and Yeast banked samples (>1e7 cells) using Prepman Ultra reagent and I seem to be struggling to obtain a sequence. Although the...
01 August 2024 2,079 0 View
I've seen articles that primarily focus on alpha and beta activity in the frontal regions, but these studies often compare healthy subjects with those having various pathologies. I haven't seen a...
31 July 2024 7,259 1 View