Protocol for IHC to stain cells cutured in 6 well or 12 well plates?

More Rana A Majid PhD's questions See All

How to extract peaklists from mass spectrometry raw file and do mascot search for my protein in ncbi?

I have a raw file of tandem mass spectrometry (LC-MS/MS) data. I want do online protein databank search for my protein identification and its homology.

30 January 2021 3,081 3 View

I get tumor tissue from the patients after the surgery. How to isolate the top layer of cells from a tumor?

I work on gastric cancer. I want to separate the top layer of tumor cells from the lower layers.

25 January 2021 6,663 1 View

Is it possible to export mesh from abaqus for using in Ansys Fluent?

Hi everyone, I want to mesh a part in Abaqus for further use in ANSYS Fluent because this way, it is much easier and faster to get a high-quality mesh. Is it even possible to generate a file...

15 January 2021 5,665 3 View

What are the (extrinsic) factors that influence the citation rate of a research article?

Dear RG members, Excluding the (intrinsic) factors such as quality and content of an article, what are the extrinsic factors/predicors that influence the citation rate of a research article? Is...

07 January 2021 3,410 3 View

Natural frequency extraction with ABAQUS?

I have modeled the RC beam with simply supported BC and I have got the frequency value for the case of the beam, but I have 150 modes for each analysis and I have to see them one by one to find...

05 January 2021 8,626 3 View

How high is the saturation electric field of P-E loop in Relaxor Ferroelectrics?

I have prepared a relaxor ferroelectric (KNN - BZZ solid solution) bulk sample. During P - E loop measurements, we experienced saturation electric field of about 100 kV/cm before dielectric break....

29 December 2020 9,080 3 View

What is the best way to simulate the fractional controllers?

What software do you prefer?

29 December 2020 889 3 View

How can i save multi medical images after some preprocessing in a file ?

i have multi x ray images after preprocessing on it , i saved them as a vector in file .dat but this file not accepted in next stage (convolution neural network ). how can i save all image in...

27 December 2020 1,369 4 View

Can anybody help to find a tool for assessing study habits of students?

It is for my research purpose

21 December 2020 833 3 View

What is microbiology and its types,scope?

the study of microorganisms is called microbiology Microbiology is the study of microscopic organisms, such as bacteria, viruses, archaea, fungi and protozoa. This discipline includes fundamental...

13 December 2020 8,287 6 View

Does Napabucasin soluble in dichloromethane and in ethanol?

I need to prepare a solution of Napabucasin (inhibitor of cancer cell stemness) in dichloromethane. The drug is expensive, so maybe somebody already tried to do it. If it is soluble, what is the...

02 March 2021 9,945 3 View

Could you please recommend some good journal that accept very long revie papers (approximately 6000+ words) in neurosurgery, cancer biology ?

01 March 2021 8,087 3 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View

Anyone know of a good progesterone antibody?

I am looking for a good progesterone antibody to quantify levels of progesterone in zebrafish. Applications would be ELISA, and immunohistochemistry.

28 February 2021 5,929 2 View

Anyone that can help with Maxquant TMT16plex which is missing?

Hi I am very new to proteomics analysis. I have got raw files from core facility running thermofisher TMT 16plex 4 hours. I cannot find on maxquant the option TMT16Plex there is only up to 11...

25 February 2021 1,675 3 View

Is it possible that Cancer cells could survive in heparinized blood for 48 hours?

Hi everyone, I am just wondering if we incubated 200 microliter of whole blood with 50 microliter of cancer cells(1bout 5000cells) do you think that cancer would survive for 48 hours?

23 February 2021 3,840 3 View

How to manage western blot for proteomics data validation?

Hi Everyone, I am a bigginer in western blotting and I am trying to use it for validation of my proteomics data. I have: two regulated proteins with molecular mass of 40 and 73 kDa one control...

23 February 2021 6,671 4 View

The PC12 cell line characteristics?

Hello dear scientists, Does the undifferentiated PC12 cell line in terms of chromosome characteristics, proliferation, and drug reactions have the characteristics of cancer cells? Where is it...

21 February 2021 8,945 3 View

What is the minimum number of exosomes/ml to have a good yield of total RNA for Illumina sequencing?

I am using IZON qEV2 columns to isolate exosomes from 100 ml of cancer cell conditioned medium. in NTA analysis I usually see concentration of 2x10^9 particles/ml. But when I proceed with this...

20 February 2021 6,879 3 View

Shreyas Lingadahalli

Abcam has good protocols on their website. It has worked for me.

Joseph Hardin

You should be able to use a standard protocol. As for antigen retrieval, you will have to use the enzymatic approach since the plates can't be immersed in antigen retrieval solution and put in a decloaker.