Protocol for DNA extraction from Soil?

More Rubiela Rosalia's questions See All

Bioinformatics approach species identification and pcr primer design. What tools can I use?

Hello. I'm doing a Bioinformatics minor and I've been given a project on species identification and primer design. With little background knowledge on what tools to use and how to review species...

18 February 2022 8,196 3 View

How to get ICRG (Economic Risk ratin) data ?

Hi, I'm a management student that want some help for finding the excel data of Economic Risk Rating from 2014 to 2019 or at least to 2018. If there's anyone who can help me please share the data...

13 January 2020 1,286 1 View

What does a System Matrix Inversion Failed an error in DigSILENT means?

Hello. I have a network with number of VSDs which I did modelled in Digsilent and getting an error of System Matrix Inversion Failed when I run a RMS/EMT study to check how the motor starts as...

31 October 2019 5,084 9 View

How much time is expected to be the limit between suffering a PSTD having been a victim of sexual abuse?

For example, if you have been abused in your childhood...it is expected you are suffering PSTD when you are a teenager or an adult? What about the brain changes regarding to this question? Is the...

14 January 2018 468 1 View

How can I input latitude and longitude as explanatory variables in GESTE 2.0 (population genomics software)?

The manual explains how to input environmental variables ~(or factors) and interactions between them but does not explicity explain how to input geographical coordinates. Should I just input...

20 January 2017 5,490 1 View

How can the GLS coefficient values be as low as 0.009 and still significan (p = 0.004)?

Hi, Im trying to analyze the relationship between morphological and ecological variables through the phylogeny of the group using BM and OU models. But I don't know how to explain that GLS...

18 August 2015 2,994 4 View

Transfer spirit in conservation building

How to transfer enthusiasm from old buildings to new buildings in conservation studies in the case of demolishing old buildings and constructing new buildings on the grounds of malfunction?

01 January 1970 985 0 View

How to learn more about SPSS and its Application?

I would like to learn more about SPSS and Its application especially in regards to data analysis. Please suggest me how I can learn more about it. Thank you so much.

11 August 2024 9,101 4 View

Is there a problem with my RNA pellet?

Hello, I am currently having problems with RNA extraction. I am using mouse liver (C57BL6J), and I have extracted RNA from mouse liver before. Before this experiment, my final RNA pellets were...

11 August 2024 7,082 3 View

Can I base on reverse DNA sequences to perform alignment, convert to amino acids and GenBank submission?

I have reverse sequences (AB1 format), can I base on reverse DNA sequences to perform nucleotide alignment, convert nucleotides to amino acids and deposit the sequence in GenBank database?

11 August 2024 5,138 1 View

Baseline drift in HPLC? What causes this?

Hello, Why do i see this baseline drift when i compare my blank (black) to the sample (blue)? Any suggestions as to why this happened? Thank you!

11 August 2024 3,770 4 View

Text-Communication from the M1 Hand Area using BCI—and then there is Elon Musk?

Willett, Shenoy et al. (2021) have developed a brain computer interface (BCI) that used neural signal collected from the hand area of the motor cortex (area M1) of a paralyzed patient. The...

10 August 2024 7,180 0 View

Has anyone applied Python in the field of textile engineering for data analysis, automation, or smart textiles?

I'm currently exploring the application of Python in textile engineering, specifically in areas like data analysis, process automation, and the development of smart textiles. I'm interested in...

10 August 2024 7,429 2 View

How can I use the cif data obtained from rietveld refinement extracted via gsas2, for microstructural analysis using ETEX software?

09 August 2024 7,718 0 View

RNA Extraction Using Hot Borate Method No Longer Working?

I've been performing RNA extraction on cotton petiole tissue for a few months now using the method described in the following paper, a derivative of the typical hot borate method...

08 August 2024 9,882 2 View

How are iso-frequency contours plotted?

Let's say we have a standard, regular hexagonal honeycomb with a 3-arm primitive unit cell (something like the figure attached; the figure is only representative and not drawn to scale). The...

07 August 2024 1,937 1 View

How to prepare the nanoparticle treated fungal sample for Environmental SEM analysis?

A fungal strain was treated with nanoparticles. We want to do an environmental SEM analysis. So could anyone share your views on preparing the sample? Thank you.

07 August 2024 5,307 1 View

Craig Carlton Dremann

Check what the project at UC Santa Cruz is doing with their CALeDNA project at https://ucedna.com/

I did some sampling a few years ago, for that project.

Rubiela Rosalia

Thank you!

The cost if I submitted the sample for the UCSC lab to run a sample for me, was less than $200. Maybe instead of setting up all the equipment yourself, have their lab run your samples?