Can someone give me detailed protocol to measure the affinity constants using non-competitive enzyme immuno-assay for monoclonal antibody?
For the detection of antigen in serum samples, I think dissociation of immune complexes formed in-vitro or in-vivo is necessary so that bound antigens can be detected. Can you please enlighten me...
01 February 2013 246 3 View
My culture grows in presence of tween 80 . I need to remove it from the culture supernatant for assaying the supernatant for various parameters. Any suggestions for removing tween?
05 June 2011 4,857 1 View
My Culture supernatant contains 1.25 % Tween 80 . I need to remove it for assaying my culture supernatant for various parameters. Any suggestions how can i do it ?
05 June 2011 8,946 5 View
I am new to real time Pcr and i am doing delta-delta ct method for relative quantification of gene expression. i wish to know that is it necessary to use the same dilution of target gene and house...
04 May 2011 5,632 3 View
how to detect pseudogene in prokaryotes???
10 November 2009 9,454 0 View
Is there any bioinformatic tool to determine pseudogene in prokaryotes???
10 November 2009 9,833 0 View
Hi all, I am validating a polyclonal antibody raised against human ANT4, through WB and ICC. In most of my controls, the antibody shows very little off-target binding, but when I pre-incubate the...
03 March 2021 5,999 3 View
Hello! I want to quantify by ELISA the secreted (from platelets poor plasma) and the non-secreted (from platelet lysate) PF4 before and after TRAP stimulation. I will use the ELISA from R&D...
03 March 2021 1,499 2 View
Santa Cruz now recommends this new antibody (F-10) but I can't find a publication using this reference. I'm working on human et non human primate hypothalamus and so far, it doesn't work. Is...
02 March 2021 10,003 2 View
If the detection range is in ng/ml but the reference range is in ug/ml for a molecule or protein in serum or plasma .how to dilute and what is the initial volume to be taken for quantitative analysis
02 March 2021 7,670 3 View
Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...
01 March 2021 3,867 3 View
We have been using Thermo Fisher anti-cardiac Troponin T antibody for immunofluorescence staining in FFPE mouse heart sections but it was recently discontinued. The literature shows it to be the...
01 March 2021 4,937 1 View
I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....
01 March 2021 3,607 3 View
I am a Petroleum Production Engg MSc student at Robert Gordon University in Aberdeen and I am in the process of selecting a topic for my MSc Engineering Investigation proposal. I would very much...
01 March 2021 7,396 4 View
I have used an AllPrep DNA/RNA/Protein Mini Kit QIAGEN kit to extract RNA and protein from my samples. At the end of the protein purification, I resuspend my protein in 5% SDS. Will these samples...
28 February 2021 7,370 3 View
Hello, I was just wondering how does a antibody have full intact antibody + half antibody? Is it because of the drug conjugation?
28 February 2021 1,577 1 View