Can someone give me detailed protocol to measure the affinity constants using non-competitive enzyme immuno-assay for monoclonal antibody?
For the detection of antigen in serum samples, I think dissociation of immune complexes formed in-vitro or in-vivo is necessary so that bound antigens can be detected. Can you please enlighten me...
01 February 2013 294 3 View
My Culture supernatant contains 1.25 % Tween 80 . I need to remove it for assaying my culture supernatant for various parameters. Any suggestions how can i do it ?
05 June 2011 9,207 5 View
My culture grows in presence of tween 80 . I need to remove it from the culture supernatant for assaying the supernatant for various parameters. Any suggestions for removing tween?
05 June 2011 4,910 1 View
I am new to real time Pcr and i am doing delta-delta ct method for relative quantification of gene expression. i wish to know that is it necessary to use the same dilution of target gene and house...
04 May 2011 5,694 3 View
how to detect pseudogene in prokaryotes???
10 November 2009 9,509 0 View
Is there any bioinformatic tool to determine pseudogene in prokaryotes???
10 November 2009 9,885 0 View
Can anyone share any reference that gives mouse specific CD3 antibody sequence. I have been searching for this since few days and no luck. Thank you very much
08 August 2024 9,157 4 View
I'm working on selecting antibodies against a recombinant protein that has a His-tag. My idea is to first bind the recombinant protein to a HisTRAP column and then use this column for an affinity...
07 August 2024 505 3 View
I am staining some brain sections stored in cryoprotectant that express a Histone H2B- GFP fusion protein that were generated ~10 years ago. I know I need to enhance signal with an anti-GFP...
07 August 2024 5,338 2 View
To compare positive and negative cell populations in flow cytometry, should I compare unstained cells with antibody stained cells? Or with the isotype control? Most papers show comparison with...
06 August 2024 6,728 6 View
Hello everyone, I am researching ways to enhance the quality of lamb milk replacer for machine feeding. I would appreciate any insights or recommendations on the following: Manual vs. Machine...
06 August 2024 6,227 2 View
I need the python code to forecast what crop production will be in the next decade considering climate and crop production variables as seen in the attached.csv file.
05 August 2024 2,977 3 View
Are there any fluorescently labeled anti-Alpaca secondary antibodies raised in Donkey? So far I have only been able to find anti-Alpaca secondaries raised in Goat. Or is this not possible due to...
04 August 2024 4,255 1 View
What are the biological importance of bacteria and the role of microbes to improve crop productivity and soil health and role of bacteria as decomposers in cycling nutrients?
31 July 2024 3,479 5 View
We have a lateral flow test on Sartorius 140 NC. The conjugate is gold-monoclonal antibody. Using different control lines (GAM, protein A, protein G) we get a very strong leading edge with reduced...
31 July 2024 4,510 3 View
What is the role of microorganisms in soil fertility and crop production cycles and role of microorganisms in mineral transformation?
31 July 2024 8,483 8 View