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Questions related from Subha Sivakolundu
Can someone give me detailed protocol to measure the affinity constants using non-competitive enzyme immuno-assay for monoclonal antibody?
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For the detection of antigen in serum samples, I think dissociation of immune complexes formed in-vitro or in-vivo is necessary so that bound antigens can be detected. Can you please enlighten me...
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My Culture supernatant contains 1.25 % Tween 80 . I need to remove it for assaying my culture supernatant for various parameters. Any suggestions how can i do it ?
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My culture grows in presence of tween 80 . I need to remove it from the culture supernatant for assaying the supernatant for various parameters. Any suggestions for removing tween?
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I am new to real time Pcr and i am doing delta-delta ct method for relative quantification of gene expression. i wish to know that is it necessary to use the same dilution of target gene and house...
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how to detect pseudogene in prokaryotes???
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Is there any bioinformatic tool to determine pseudogene in prokaryotes???
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