Protocol for azocasein zymography?

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G_mmpbsa-1.7.1 installation ?

I have used g_mmpbsa (rashmi kumari version) for mmpbsa study in my previous simuation. Everything was ok. But i performed three simulations on cluster having gromacs 2021. Now the .xtc and .tpr...

20 May 2024 6,208 1 View

Articles on information processing apporach in second language acquisition ?

I need a research article on information processing in second language acquisition or any article that contains at least one approach of learning or acquiring second language acquisition.

13 December 2023 7,920 2 View

How to calculate total sugar utilization, ethanol yield g/g and ethanol yield g/l from fermentation broth?

I am working on bioethanol production from lignocellulosic biomass. However, I require some clarification on the methods for calculating initial sugar concentration, sugar consumption rate,...

20 September 2023 4,435 2 View

Adsorption study of naproxen sodium?

I'm doing an adsorption study of naproxen sodium. The previously published articles mentioned that naproxen sodium shows optimum adsorption at pH 4-6, and as the pH increases, the adsorption...

07 August 2023 2,380 3 View

Why my h index not showing and my citation is also not showing?

why my h index not showing and my citation is also not showing?

14 June 2023 8,194 1 View

How to carry out adsorption kinetic experiment? kindly let me know in details. I wanna check adsorption kinetic of my composite for dye degradation?

Kindly let me know in detail. I wanna check the adsorption kinetic of my composite for dye degradation.

05 June 2023 5,584 3 View

Which tools or methods are used to compare the relative gene expression of real time pcr value other than Livak method??

Which tools or methods are used to compare the relative gene expression of real-time PCR value other than the Livak method?? I have values of CQ of both gene and internal reference. I want to know...

30 May 2023 6,235 2 View

How to convert cellulase 13000 CMC U/g (commercial product) into FPU/ml?

I have the commercial cellulase product (100mg) with 13000 U/g. I need to know how to convert this U/g units into FPU unit for saccharification process with lignocellulosic biomass. Thanks in advance.

19 March 2023 6,383 1 View

How to do ethanol productivity calculations from fermentation broth with pure glucose and xylose as substrate?

I did ethanol production using 50g/L of glucose and xylose in fermentation broth. Now need to find ethanol productivity, their theoretical yield and fermentation efficiency, also consumption of...

01 March 2023 5,195 8 View

How to remove or reduce water content in the medium for ethanol quantification through GC-FID?

The conversion of sugars into bioethanol occurred in the fermentation medium. However for GC analysis, in capillary or packed column, water is biggest enemy for instrument. still in need of...

31 January 2023 7,615 2 View

Can I use a HisTRAP column for affinity chromatography?

I'm working on selecting antibodies against a recombinant protein that has a His-tag. My idea is to first bind the recombinant protein to a HisTRAP column and then use this column for an affinity...

07 August 2024 505 3 View

How do soil microflora interact with plant roots and influence plant nutrition, health, and productivity?

06 August 2024 9,618 3 View

What is the best blank for nanodrop if I want to read a recombinant protein concentration?

Is it the "elution buffer" or the "dialysis buffer"? Note: I'll be using NanoDrop OneC

01 August 2024 967 3 View

How do soil microbes affect plant health and productivity and how do plants interact with soil microorganisms and contribute to soil fertility?

31 July 2024 5,087 4 View

What is the role of decomposers in the cycling of matter in the biosphere and role do soil microbes play in plant nutrition availability and uptake?

31 July 2024 7,921 5 View

How microorganisms are important for maintaining of healthy soil and biodiversity and microorganisms and plant roots contribute to soil formation?

31 July 2024 8,939 5 View

Problems with ion exchange chromatography?

I'm having problems with ion exchange chromatography. After applying my sample (a fluorescent protein), a preferential path inevitably forms. The matrix I use is DEAE-Sephacel. Could someone...

27 July 2024 6,605 0 View

How can biogenic synthesis techniques be applied to develop plants capable of extracting and processing heavy metals from contaminated soil, and wha?

Understand how to utilize biosynthesis to develop natural and sustainable solutions to address heavy metal pollution and improve environmental and agricultural conditions.

26 July 2024 1,469 1 View

How do regenerate Amberlite XAD 4 resin for further cycles of use for removing 0.5% Sarkosyl from recombinant protein solutions?

I use 0.5% sarkosyl to solubilise recombinant proteins contained in Inclusion bodies of E. coli. sarkosyl very effeciently solubilizes proteins from Inclusion bodies and I am trying to use...

25 July 2024 5,001 2 View

Can I separate xylene from paraffin after deparaffinization of microtome samples and reuse the xylene?

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24 July 2024 1,559 1 View