Hello Researchers, can organizational socialization effects on organizational ambidexterity? what's your opinion?

organizational ambidexterity simply talks about explosive and explorative innovation and how can this variable used with different variables i.e. leadership, resources, organization antecedent....

28 December 2020 4,022 3 View

Comparing correlated means in a t-test?

Hello, I have a categorical variable (main teaching assignment) with 12 options (early childhood, mathematics, English, etc) and I am trying to determine which groups earn significantly higher...

08 September 2020 5,046 5 View

How to grow a difficult breast cancer cell line?

Here I am trying to optimizing the growth of breast cancer cell which is ++- requiring additional growth factors (20% FBS, insulin and EGF) for its growth. After adding the required growth factors...

20 August 2019 4,575 2 View

LC/ESI/MS program for tryptic in solution digest?

Can anyone provide me the proper program for LC/ESI/MS for tryptic digest in solution. I have literature surveyed and found different suggestions. In some cases, 100 % acetonitrile 0-40 % for...

30 May 2018 8,848 2 View

The elastic constants calculation on graphene gives me the value 115GPa. But the correct value is 1TPa. How can I correct this?

The elastic constants of graphene are calculated using castep code. I am getting the value of C11 as 115GPa and youngs modulus as 112 GPa. But the reported value is 1TPa. I do not know where i am...

21 January 2016 3,747 1 View

How do I completely remove DNA from RNA sample, without degrading it?

09 November 2015 7,617 4 View

What happens if concentration of acrylamide is increased?

I accidently added double volume of bisacrylamide i.e: 1.6 instead of 0.8 during the preparation of solution. Will it effect the sds page or protein bands? Please suggest me. Thank You..........

28 March 2015 1,510 12 View

Is anyone familiar with zymogram protein sampling?

I ran a zymogram of protein samples but couldn't get the result. The gel was half stained and half destained showing no zone of hydrolysis. Why is it so? The protein zymogram was run but the gel...

25 November 2014 8,986 18 View

Is anyone interested in collaborating on the ecological importance of specific tree species?

07 July 2014 2,862 4 View

How can I compute the total frequency of an image in MATLAB?

I am doing my project in MATLAB and doing a remote sensing area. I am using a split and merge method for segmentation and in that algorithm, using the total frequency of an image.

26 March 2014 534 6 View

What kind of fluid could I use for a pitfall trap that also does not invalidate molecular testing?

Hi, I want to start testing pitfall trap to obtain ants samples, but I need to conduct molecular analysis on those insects. So, what kind of fluid can I use? Ethanol expires too early and I need...

03 March 2021 5,978 5 View

Buffer Exchange using column packed with sephadex G-25 effect on protein concentration?

Hello, If i am doing a buffer exchange for an antibody of 1mg/mL, does the elution lose protein in the process of buffer exchange? For example, if i flow through 500 uL of 1mg/mL sample, and...

03 March 2021 6,299 3 View

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03 March 2021 5,360 2 View

Waters 2707 HPLC autosampler problem?

I am using a 2707 waters HPLC device. When I try to inject a sample, it says missing plate or rack. I changed the needle and calibrated its position but I still get the same problem. I even get...

02 March 2021 1,408 1 View

How to quantitative analize the data of TNBS assay for gelatin?

Estemeed colleagues, I found some issues regarding the quantification of the data for TNBS assay. There are different protocols on how to perform that but it is clear to me the "fil rouge" that...

02 March 2021 2,616 1 View

Does Napabucasin soluble in dichloromethane and in ethanol?

I need to prepare a solution of Napabucasin (inhibitor of cancer cell stemness) in dichloromethane. The drug is expensive, so maybe somebody already tried to do it. If it is soluble, what is the...

02 March 2021 9,945 3 View

How optimize the Size Exclusion Chromatography?

Hello! I'll do a size exclusion chromatography, but I only have an open column, and I'll perform the peptide extraction from yeast, using buffer lysis (sodium phosphate 50 mM/NaCl 30 mM/DNAse and...

01 March 2021 2,215 2 View

How to accurately quantify chitin?

Hi all, I have been working on the quantification of chitin in insect samples and feed for a while but I can notice that the quantification methods (e.g. exposure to HCl, NaOH, and weighing sample...

01 March 2021 1,843 2 View

Precipitate after adding laemmli buffer to protein solution containing Potassium Acetate?

Hi, I am running a size exclusion chromatography experiment with a buffer containing Potassium Acetate as a salt. I analyse these fractions through SDS-PAGE. After boiling my SEC fractions in...

01 March 2021 2,622 3 View

Any research papers for extraction of nutrient deprivation on macroalgae? Analysed by HPLC-UV and GC/MS. Possibly from macroalgae genus Rhizoclonium?

I am doing my thesis on the nutrient deprivation on the macroalgae Rhizoclonium sp. I will be analysing the levels over a period of roughly 5 days by HPLC-UV and GC/MS. I will be using seawater as...

28 February 2021 6,477 1 View