How to convert cellulase 13000 CMC U/g (commercial product) into FPU/ml?

20 March 2023 1 6K Report

I have the commercial cellulase product (100mg) with 13000 U/g. I need to know how to convert this U/g units into FPU unit for saccharification process with lignocellulosic biomass. Thanks in advance.

Nick Gee

You have 13000 U/g. Is that dry powder or liquid? If it is powder you need to dissolve it in a known final volume of liquid. As you have 100mg, you have 1300 units of enzyme and the units per ml value is determined by the volume. If you have 100mg/1300 units dissolved in 10ml, i.e., 10mg/ml, that’s 130 units per ml. But if you had 1mg/ml enzyme (100ml), you would have 13units/ml.

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