Can you recommend a tutorial for NGS data analysis?

I'm trying to learn to interpret the data obtained from NGS step by step. Where can I find information or a tutorial?

10 November 2013 2,876 6 View

Gel electrophoresis using a ladder of 1 Kb

I'm using a new ladder of fermentas for the visualization of big size fragments. This ladder starts in 250 pb and ends in 10 kb and I need a perfect separation of the bands in a gel of 0.8 %. Has...

09 October 2012 5,054 15 View

Amplification by conventional PCR of large fragments

I need to find an article who talks about amplification by conventional PCR of fragments of big size... 2 or 3 Kb if anyone knows about it. Thanks

05 June 2012 7,473 9 View

Phusion Polymerase

Recently I've been trying to amplify 4kb using the phusion polymerase, but I have doubts with the annealing temperature, can somebody recomend an article talking about that. and give me some advice?

04 May 2012 8,348 8 View

Amplification of fragmets bigger than 3 KB

Hello everyone , recently i'll trying amplify by PCR a fragment of 5 kb .... anyone knows articles talking about that ... .without use long PCR kit? is posible? thanks!

31 December 2011 7,659 7 View

Hi everyone! I need your help for download an article.... please i need it :) thanks all

Protection from Endogenous Perforin: Glycans and the C Terminus Regulate Exocytic Trafficking in Cytotoxic Lymphocytes Immunity. 2011 Jun 24;34(6):879-92. Epub 2011 Jun 9. Brennan AJ, Chia J,...

31 December 2011 7,749 6 View

next Generation sequencing

Hello everyone I wanna learn about the procedures and mechanisms .... in this area ...in where can i read o something -.... thanks

11 December 2011 1,684 6 View

Ensemble database vs ncbi

Hi everyone, i have a question...recently found de Ensemble DAtabase and comparing the sequences in the NCBI are diferent for the same gene ....in which can i trust? ...i always use the NCBI but...

10 November 2011 3,079 12 View

error in running a model in Abaqus

Excessive rotation of nodes in node set ErrNodeExcessRotation-Step1

17 August 2021 0 0 View

error in modelling model in Abaqus

Excessive rotation of nodes in node set ErrNodeExcessRotation-Step1

17 August 2021 0 0 View

MD MOE RMSD

How to calculate the RMSD values for a MD simulation using MOE?

07 August 2021 0 0 View

MD MOE RMSD

How to calculate the RMSD values for a MD simulation using MOE?

07 August 2021 0 0 View

MGL tools

20 June 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

i have some problem with chtMultiRegionSimpleFoam

--> FOAM FATAL ERROR: Maximum number of iterations exceeded From function Foam::scalar Foam::species::thermo::T(Foam::scalar, Foam::scalar, Foam::scalar, Foam::scalar (Foam::species::thermo::*)(Foam::scalar, Foam::scalar) const, Foam::scalar (Foam::species::thermo::*)(Foam::scalar, Foam::scalar) const, Foam::scalar (Foam::species::thermo::*)(Foam::scalar) const) const [with Thermo = Foam::hConstThermo >; Type = Foam::sensibleEnthalpy; Foam::scalar = double; Foam::species::thermo = Foam::species::thermo >, Foam::sensibleEnthalpy>] in file /home/ubuntu/OpenFOAM/OpenFOAM-4.1/src/thermophysicalModels/specie/lnInclude/thermoI.H at line 66. FOAM aborting #0 Foam::error::printStack(Foam::Ostream&) at ??:? #1 Foam::error::abort() at ??:? #2 Foam::heRhoThermo >, Foam::sensibleEnthalpy> > > >::calculate() at ??:? #3 Foam::heRhoThermo >, Foam::sensibleEnthalpy> > > >::correct() at ??:? #4 ? at ??:? #5 __libc_start_main in "/lib/x86_64-linux-gnu/libc.so.6" #6 ? at ??:?

24 March 2021 0 0 View