Protein Topology - FAQS.TIPS

More Camilo Andres Perez's questions See All

Can you recommend a tutorial for NGS data analysis?

I'm trying to learn to interpret the data obtained from NGS step by step. Where can I find information or a tutorial?

10 November 2013 2,931 6 View

Gel electrophoresis using a ladder of 1 Kb

I'm using a new ladder of fermentas for the visualization of big size fragments. This ladder starts in 250 pb and ends in 10 kb and I need a perfect separation of the bands in a gel of 0.8 %. Has...

09 October 2012 5,115 15 View

Amplification by conventional PCR of large fragments

I need to find an article who talks about amplification by conventional PCR of fragments of big size... 2 or 3 Kb if anyone knows about it. Thanks

05 June 2012 7,549 9 View

Phusion Polymerase

Recently I've been trying to amplify 4kb using the phusion polymerase, but I have doubts with the annealing temperature, can somebody recomend an article talking about that. and give me some advice?

04 May 2012 8,406 8 View

Amplification of fragmets bigger than 3 KB

Hello everyone , recently i'll trying amplify by PCR a fragment of 5 kb .... anyone knows articles talking about that ... .without use long PCR kit? is posible? thanks!

31 December 2011 7,721 7 View

Hi everyone! I need your help for download an article.... please i need it :) thanks all

Protection from Endogenous Perforin: Glycans and the C Terminus Regulate Exocytic Trafficking in Cytotoxic Lymphocytes Immunity. 2011 Jun 24;34(6):879-92. Epub 2011 Jun 9. Brennan AJ, Chia J,...

31 December 2011 7,812 6 View

next Generation sequencing

Hello everyone I wanna learn about the procedures and mechanisms .... in this area ...in where can i read o something -.... thanks

11 December 2011 1,747 6 View

Ensemble database vs ncbi

Hi everyone, i have a question...recently found de Ensemble DAtabase and comparing the sequences in the NCBI are diferent for the same gene ....in which can i trust? ...i always use the NCBI but...

10 November 2011 3,151 12 View

Which Scopus Journal provides the most affordable fees?

"PUBLISHING IN A SCOPUS JOURNAL" Researchers are now at a cross road. The critical need to publish in a Scopus or ISI, etc journal is ever vital. Journal Publication fees must be submitted....

10 August 2024 8,621 1 View

Seeking Advice on Viability and Execution of Undergraduate Thesis Topic?

Hello everyone, I am currently developing a thesis proposal and would appreciate your input on its viability and how to effectively carry it out. My proposed topic is: "Does the perceived threat...

10 August 2024 8,992 0 View

Who will be moral responsible for the death of thousands of people in the event of an earthquake?

Who will bear moral responsibility for the deaths of thousands of people in the event of an earthquake? Weeks and months remain before the onset of strong earthquakes that bring death to...

08 August 2024 6,134 12 View

Are there any instruments for studying time similar to the way it is in space?

There are a huge number of methods for studying objects in space, according to the senses (and not only). Mechanical, thermal, optical, acoustic, electrical, magnetic, based on particle beams,...

06 August 2024 7,102 0 View

Weak DAPI staining after immunohistochemistry - how to improve?

After immunohistochemistry of previously fixed in PFA and EtOH and then frozen 20 μm sections of zebrafish brain, DAPI staining is very weak (right) compared to the same sections stained without...

05 August 2024 9,637 2 View

Why did the authors extrapolate a phenotype that they experimentally proved in one bacterial strain across the whole genus of the organism?

I aim to be as skeptical as possible regarding whether a pair of orthologous genes results in the same phenotype in their different but related bacterial organisms under similar environmental...

05 August 2024 6,787 4 View

The Curse of Evolution and Complexity?

Brain and body mass together are positively correlated with lifespan (Hofman 1993). The duration of neural development is one of the best predictors of brain size, and conception is the best...

05 August 2024 6,247 3 View

In the case of a wound l recurrence after radical breast cancer and sentinel lymph node biopsy. Are the sentinel lymph node procedure recommended?

In the case of a wound l recurrence after radical breast cancer and sentinel lymph node biopsy. Are the sentinel lymph node procedure recommended? If no axillary lymph node dissection was not...

05 August 2024 8,056 1 View

Regarding a model for simulating battery charge and discharge, what do you consider to be high fidelity?

Regarding a model for simulating battery charge and discharge, what do you consider to be high fidelity? What is the acceptable percentage of error (regardless of the metric)? Could you suggest...

03 August 2024 5,358 0 View

Interested in a SCOPUS collaboration?

Hi RG family. My team and I are working on some SCOPUS publications and we need co-authors who are willing and capable of undertaking both qualitative and quantitative-based studies. The scope...

02 August 2024 7,843 0 View

Peter Balázs Kós

RASMOL can be the first to try of the very many, although if you are not careful enough, the web servers you use for the PDB things will inject some pulgins into your browser too.

Bret Heale

Concur easy to use and can be linked easily with homology modeling

Camilo Andres Perez

but for made the topology in 2D i can use any of them?

Can i*

Matej Lexa

There is a program called "topdraw", where you can create these yourself and PDBsum contains topology drawings for PDB proteins (e.g. http://www.ebi.ac.uk/thornton-srv/databases/cgi-bin/pdbsum/GetPage.pl?pdbcode=1a06&template=protein.html&r=wiring&l=1&chain=A). Other than that, I don't know. Does anybody know a program that draws these automatically from PDB data?