Dear all,

I want to know the structural changes of a protein in various solvents using CD technique. Then, questions come:

(1) Buffer baselines: I would like to check the structures of my protein in 0.5 M NaCl solvent , 30% EtOH, as well as in other organic solvents. While as I know CD measurements require low ionic solvents, and certain ions are not preferred. I planned to subtract the results using individual buffer baselines. Now, I'm wandering the quality of my data, as HT values in some buffers are quite high when the wavelength is less than 200 nm. A simple subtraction would be ok? Is the data trustable?

(2) Would you like to recommend some other better techniques to check the structures of proteins in various solvents, including both organic ones and the ones with high ionic strength.

Any feedbacks and discussions are appreciated. Thanks in advance.

Best,

Yuhong